GERHARDT P, BLACK S H
J Bacteriol. 1961 Nov;82(5):750-60. doi: 10.1128/jb.82.5.750-760.1961.
Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be dependent on at least three molecular properties: (i) The dissociation of electrolytes usually resulted in high or low uptake predictable from their charge. (ii) Lipid insolubility restricted permeation of small molecules. (iii) The molecular weight of unsubstituted glycol and sugar polymers exponentially limited penetration to eventual exclusion at mol wt above 160,000. The results were plotted as a generalized curve, calculations from which permitted an interpretation that the effective spore surface contains pores varying in diameter from 10 to 200 A.
格哈特，菲利普（密歇根大学，安阿伯分校）和S. H. 布莱克。细菌芽孢的渗透性。II. 影响溶质渗透的分子变量。《细菌学杂志》82:750 - 760。1961年。——对100多种化合物进行了测试，以检测它们被一种芽孢杆菌的休眠芽孢摄取的情况。发现渗透程度至少取决于三种分子特性：（i）电解质的解离通常导致根据其电荷可预测的高摄取或低摄取。（ii）脂溶性差限制了小分子的渗透。（iii）未取代的二醇和糖聚合物的分子量以指数方式限制渗透，直至分子量超过160,000时最终完全排除。结果绘制成一条通用曲线，据此进行的计算允许一种解释，即有效的芽孢表面含有直径从10到200埃不等的孔。
