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The effect of temperature change on the kinetic behaviour of yeast phosphofructokinase.

作者信息

Nissler K, Schellenberger W, Wolf J, Hofmann E

出版信息

Acta Biol Med Ger. 1976;35(10):1273-7.

PMID:139067
Abstract

Yeast phosphofructokinase does not exhibit any cold sensitivity. The kinetic properties of the enzyme have been investigated in the range between 10 degrees C and 30 degrees C in dependence on fructose 6-phosphate and ATP. Although a significant increase in the enzyme activity with rising temperature does not occur, the shape of the ATP velocity curves is not markedly altered. With increasing concentrations of fructose-6-phosphate the efficiency of temperature on the catalytic process increases, indicating a small temperature effect on the shape of the fructose-6-phosphate velocity curves. The results are interpreted in terms of an adequate kinetic model.

摘要

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