二磷酸腺苷四磷酸（Ap4A）通过肾上腺嗜铬细胞中一种假定的P2Y嘌呤受体介导的钙离子储存动员
Ca(2+)-stores mobilization by diadenosine tetraphosphate, Ap4A, through a putative P2Y purinoceptor in adrenal chromaffin cells.
作者信息
Castro E, Pintor J, Miras-Portugal M T
机构信息
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, España.
出版信息
Br J Pharmacol. 1992 Aug;106(4):833-7. doi: 10.1111/j.1476-5381.1992.tb14421.x.
Abstract
- Diadenosine tetraphosphate (Ap4A) evoked a concentration-dependent increase in cytosolic [Ca2+] in resting chromaffin cells. The EC50 value for this action was 28.2 +/- 6.6 microM. This effect was also produced by diadenosine pentaphosphate (Ap5A) with an EC50 of 50 +/- 7 microM. 2. In contrast with this effect, pretreatment with Ap4A or Ap5A induced a 30% reduction in Ca2+ entry following 10 microM dimethylphenylpiperazinium. 3. The elevation in cytosolic [Ca2+] induced by Ap4A was persistent in approximately 100 nM external [Ca2+] and was sensitive to depletion of internal Ca2+ stores by a bradykinin prepulse or whole cell depletion in Ca2+. 4. The effect of Ap4A was mimicked and desensitized by the agonist adenosine 5'-O-(2-thiodiphosphate), and blocked by the P2Y-receptor antagonist, cibachrome blue. The P2X-receptor agonist alpha,beta-methylene adenosine 5'-triphosphate was inactive both by itself or in combination with Ap4A. This is compatible with a P2Y-purinoceptor-mediated action.
摘要
- 四磷酸二腺苷（Ap4A）可使静息嗜铬细胞胞质内的[Ca2+]浓度呈浓度依赖性升高。此作用的半数有效浓度（EC50）值为28.2±6.6微摩尔。五磷酸二腺苷（Ap5A）也产生此效应，其EC50为50±7微摩尔。2. 与该效应相反，用Ap4A或Ap5A预处理可使10微摩尔二甲基苯基哌嗪作用后Ca2+内流减少30%。3. 在细胞外[Ca2+]约为100纳摩尔时，Ap4A诱导的胞质[Ca2+]升高持续存在，且对缓激肽预脉冲或全细胞Ca2+耗竭所致的细胞内Ca2+储存耗竭敏感。4. 激动剂5'-O-(2-硫代二磷酸)腺苷模拟并使Ap4A的效应脱敏，P2Y受体拮抗剂锡巴铬蓝可阻断该效应。P2X受体激动剂α,β-亚甲基腺苷5'-三磷酸单独或与Ap4A联合使用均无活性。这与P2Y嘌呤受体介导的作用相符。
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本文引用的文献
1
HeLa cell DNA polymerase alpha is tightly associated with tryptophanyl-tRNA synthetase and diadenosine 5',5"'-P1,P4-tetraphosphate binding activities.海拉细胞DNA聚合酶α与色氨酰-tRNA合成酶以及二腺苷5',5"'-P1,P4-四磷酸结合活性紧密相关。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):838-42. doi: 10.1073/pnas.78.2.838.
2
Diadenosine triphosphate (Ap3A) mediates human platelet aggregation by liberation of ADP.三磷酸二腺苷（Ap3A）通过释放二磷酸腺苷介导人类血小板聚集。
Biochem Biophys Res Commun. 1984 Feb 14;118(3):704-9. doi: 10.1016/0006-291x(84)91451-7.
3
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Nucleic Acids Res. 1984 Feb 10;12(3):1609-19. doi: 10.1093/nar/12.3.1609.
4
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5
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Proc Natl Acad Sci U S A. 1983 Aug;80(16):4931-5. doi: 10.1073/pnas.80.16.4931.
6
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8
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