致病性酵母星状念珠菌ATCC 20408（II型）菌株的D-甘露聚糖结构，与星状念珠菌ATCC 36232（I型）菌株的结构比较。

Structure of the D-mannan of the pathogenic yeast, Candida stellatoidea ATCC 20408 (type II) strain, in comparison with that of C. stellatoidea ATCC 36232 (type I) strain.

作者信息

Kobayashi H, Takaku M, Nishidate Y, Takahashi S, Takikawa M, Shibata N, Suzuki S

机构信息

Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Japan.

出版信息

Carbohydr Res. 1992 Jul 2;231:105-16. doi: 10.1016/0008-6215(92)84012-h.

DOI:10.1016/0008-6215(92)84012-h

PMID:1394307

Abstract

Acid treatment of the cell-wall D-mannas of Candida stellatoidea strains ATCC 36232 (Type I, A3 strain) and ATCC 20408 (Type II, A2 strain) gave (1----2)-linked beta-D-manno-oligosaccharides (dp 2-5), whereas treatment with alkali gave the (1----2)-linked alpha-D-mannobiose. Conventional acetolysis of the acid- and alkali-treated D-mannan of the A3 strain gave oligosaccharides consisting of (1----2)- and (1----3)-linked alpha-D-mannopyranose residues, similar to those of Candida albicans serotype B strain. Mild acetolysis of the acid- and alkali-treated D-mannan of the A2 strain gave higher oligosaccharides that were digested by the Arthrobacter GJM-1 strain exo-alpha-D-mannosidase. The results of 1H- and 13C-NMR analyses indicated this D-mannan to contain branches with the following structures: beta-D-Manp-(1----2)-alpha-D-Manp-(1----2)-alpha-D-Manp++ +-(1----2)-alpha-D-Manp- (1----2)-D-Man, beta-D-Manp-(1----2)-beta-D-Manp-(1----2)-alpha-D-Manp -(1----2)- alpha-D-Manp-(1----2)-D-Man, and beta-D-Manp-(1----2)-beta-D-Manp-(1----2)-beta- D-Manp-(1----2)-alpha-D-Manp-(1----2)-alpha-D-Manp-(1- ---2)-alpha-D-Manp- (1----2)-D-Man, in common with the D-mannans of C. albicans serotype A strains.

摘要

用酸处理星状念珠菌菌株ATCC 36232（I型，A3菌株）和ATCC 20408（II型，A2菌株）的细胞壁D-甘露聚糖，得到了（1→2）连接的β-D-甘露寡糖（聚合度2-5），而用碱处理则得到了（1→2）连接的α-D-甘露二糖。对A3菌株经酸和碱处理的D-甘露聚糖进行常规乙酰解，得到了由（1→2）和（1→3）连接的α-D-甘露吡喃糖残基组成的寡糖，类似于白色念珠菌B血清型菌株的寡糖。对A2菌株经酸和碱处理的D-甘露聚糖进行温和乙酰解，得到了更高的寡糖，这些寡糖可被节杆菌GJM-1菌株的外切α-D-甘露糖苷酶消化。1H-和13C-NMR分析结果表明，这种D-甘露聚糖含有具有以下结构的分支：β-D-Manp-（1→2）-α-D-Manp-（1→2）-α-D-Manp+++ -（1→2）-α-D-Manp-（1→2）-D-Man、β-D-Manp-（1→2）-β-D-Manp-（1→2）-α-D-Manp -（1→2）-α-D-Manp-（1→2）-D-Man以及β-D-Manp-（1→2）-β-D-Manp-（1→2）-β-D-Manp-（1→2）-α-D-Manp-（1→2）-α-D-Manp-（1→2）-α-D-Manp-（1→2）-D-Man，这与白色念珠菌A血清型菌株的D-甘露聚糖相同。