白色念珠菌（血清型A）菌株细胞壁甘露聚糖的结构研究

Structural study of cell wall mannan of a Candida albicans (serotype A) strain.

作者信息

Kobayashi H, Shibata N, Osaka T, Miyagawa Y, Ohkubo Y, Suzuki S

机构信息

Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.

出版信息

Phytochemistry. 1992 Apr;31(4):1147-53. doi: 10.1016/0031-9422(92)80250-i.

DOI:10.1016/0031-9422(92)80250-i

PMID:1368047

Abstract

The structure of the mannan of Candida albicans NIH A-207 strain (serotype A) was investigated by adopting mild acetolysis followed by enzymolysis with an Arthrobacter GJM-1 exo-alpha-mannosidase. The resultant oligosaccharides, from pentaose to octaose (where manp = D-mannopyranose), were identified as manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)- manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp and manp beta (1----2)manp beta (1----2)manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, respectively. Analyses of alpha-linked oligosaccharides obtained by acetolysis under conventional conditions gave the same oligosaccharides, from biose to heptaose, as those obtained from the mannans of C. albicans NIH B-792 (serotype B) and J-1012 (serotype A, formerly serotype C).

摘要

采用温和乙酰解，随后用节杆菌GJM - 1外切α - 甘露糖苷酶进行酶解，研究了白色念珠菌NIH A - 207菌株（血清型A）甘露聚糖的结构。所得的从戊糖到辛糖的寡糖（其中manp = D - 甘露吡喃糖）分别被鉴定为manpβ(1→2)manpα(1→2)manpα(1→2)manpα(1→2)manp、manpβ(1→2)manpβ(1→2)manpα(1→2)manpα(1→2) - manpα(1→2)manp、manpβ(1→2)manpβ(1→2)manpβ(1→2)manpα(1→2)manpα(1→2)manpα(1→2)manp和manpβ(1→ < /sup>2)manpβ(1→2)manpβ(1→2)manpβ(1→2)manpα(1→2)manpα(1→2)manpα(1→2)manp。对在常规条件下通过乙酰解获得的α - 连接寡糖的分析表明，从二糖到庚糖得到的寡糖与从白色念珠菌NIH B - 792（血清型B）和J - 1012（血清型A，原血清型C）的甘露聚糖中得到的寡糖相同。