精子激活肽V型（SAP-V），是精子激活肽家族的第五个成员，从阿氏心海胆（Brissus agassizii）的卵条件培养液中纯化得到。

Sperm-activating peptide type-V (SAP-V), a fifth member of the sperm-activating peptide family, purified from the egg-conditioned media of the heart urchin Brissus agassizii.

Yoshino K, Takao T, Shimonishi Y, Suzuki N

Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.

Comp Biochem Physiol B. 1992 Aug;102(4):691-700. doi: 10.1016/0305-0491(92)90065-y.

Abstract

A novel type of sperm-activating peptide named sperm-activating peptide type-V (SAP-V) was isolated from the egg-conditioned media (egg jelly) of the heart urchin Brissus agassizii and the primary structure of the peptide was determined by fast atom bombardment mass spectrometry as follows: Gly-Cys-Glu-Gly-Leu-Phe-His-Gly-Met-Gly-Asn-Cys. 2. SAP-V and [Met(O)9]SAP-V stimulated the respiration of B. agassizii spermatozoa with half-maximal concentrations of 0.5 and 0.3 nM, respectively. However, half-maximal stimulation of the sperm respiration required 40 nM of S-carboxymethylated SAP-V. 3. SAP-V induced significant increases in the cyclic AMP and cyclic GMP levels in B. agassizii spermatozoa in a concentration-dependent manner. 4. The addition of SAP-V to B. agassizii spermatozoa resulted in a mobility shift of a major sperm protein (mol. wt from 133,000 to 129,000) on sodium dodecyl sulfate-polyacrylamide gels.

摘要

从阿氏心海胆（Brissus agassizii）的卵条件培养液（卵胶）中分离出一种新型精子激活肽，命名为V型精子激活肽（SAP-V），并通过快原子轰击质谱法确定该肽的一级结构如下：甘氨酸-半胱氨酸-谷氨酸-甘氨酸-亮氨酸-苯丙氨酸-组氨酸-甘氨酸-甲硫氨酸-甘氨酸-天冬酰胺-半胱氨酸。2. SAP-V和[甲硫氨酸（O）9]SAP-V分别以0.5 nM和0.3 nM的半数最大浓度刺激阿氏心海胆精子的呼吸。然而，精子呼吸的半数最大刺激需要40 nM的S-羧甲基化SAP-V。3. SAP-V以浓度依赖性方式显著提高阿氏心海胆精子中环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）的水平。4. 向阿氏心海胆精子中添加SAP-V导致十二烷基硫酸钠-聚丙烯酰胺凝胶上一种主要精子蛋白（分子量从133,000变为129,000）的迁移率发生变化。