Georgakopoulos T, Thireos G
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece.
EMBO J. 1992 Nov;11(11):4145-52. doi: 10.1002/j.1460-2075.1992.tb05507.x.
When yeast cells are grown under conditions of amino acid limitation, transcription of amino acid biosynthetic genes is increased through the action of the GCN4 transcriptional regulator. gcn5 mutant strains exhibit poor growth under such conditions. We have established that GCN4 requires the function of GCN5 in order to promote normal levels of transcriptional activation. In addition, we have shown that GCN5 is also required for the activity of the HAP2--HAP3--HAP4 transcriptional activation complex, which mediates the transcription of genes involved in respiratory functions. Thus, GCN5 is a new member of the recently revealed general class of transcriptional regulators that collaborate with certain specific DNA binding activators to promote high levels of transcription. We have cloned and sequenced the GCN5 gene. The deduced GCN5 protein contains a region conserved in other yeast, Drosophila and human proteins, all members of this new class of transcriptional activators.
当酵母细胞在氨基酸限制条件下生长时，通过GCN4转录调节因子的作用，氨基酸生物合成基因的转录会增加。gcn5突变株在这种条件下生长不良。我们已经确定GCN4需要GCN5的功能才能促进正常水平的转录激活。此外，我们还表明，HAP2-HAP3-HAP4转录激活复合物的活性也需要GCN5，该复合物介导参与呼吸功能的基因的转录。因此，GCN5是最近发现的一类转录调节因子的新成员，这类调节因子与某些特定的DNA结合激活剂协同作用以促进高水平的转录。我们已经克隆并测序了GCN5基因。推导的GCN5蛋白包含一个在其他酵母、果蝇和人类蛋白质中保守的区域，这些蛋白质都是这类新的转录激活剂的成员。
