Ma J, Ptashne M
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Cell. 1987 Oct 9;51(1):113-9. doi: 10.1016/0092-8674(87)90015-8.
We describe yeast transcriptional activators encoded by E. coli genomic DNA fragments fused to the coding sequence of the DNA-binding portion of GAL4. All of the new activating sequences that we have analyzed, like those of GAL4 and GCN4, are acidic; most of these sequences show no obvious sequence homology when compared with the identified activating regions of GAL4 and GCN4 or among themselves. We also describe a fusion protein that contains no yeast protein sequence but activates transcription in yeast.
我们描述了由与GAL4 DNA结合部分编码序列融合的大肠杆菌基因组DNA片段所编码的酵母转录激活因子。我们分析的所有新激活序列，与GAL4和GCN4的激活序列一样，都是酸性的；与GAL4和GCN4已确定的激活区域相比，或者这些新激活序列之间相互比较时，大多数序列没有明显的序列同源性。我们还描述了一种不含酵母蛋白序列但能在酵母中激活转录的融合蛋白。
