Kidou S, Tsukamoto S, Kobayashi S, Ejiri S
Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, Japan.
FEBS Lett. 1998 Sep 4;434(3):382-6. doi: 10.1016/s0014-5793(98)01014-x.
We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex with GST-eEF-1Bbeta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alphabeta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome.
我们分离出一个水稻cDNA克隆（refg），它编码翻译延伸因子1B的γ亚基（eEF-1Bγ；旧称EF-1γ）。refg编码一个由419个氨基酸组成的开放阅读框，与动物和酵母的相应序列具有相似性。复合物形成分析表明，refg的重组蛋白（His-eEF1Bγ）与GST-eEF-1Bβ形成了复合物，这表明refg编码eEF1Bαβγ复合物中的水稻eEF1Bγ。表达分析表明，refg mRNA在悬浮培养细胞生长的指数期非常丰富。DNA印迹分析表明，refg位于水稻基因组的一个单一基因座上。
