编码水稻延伸因子1β的cDNA的克隆与特性分析
Cloning and characterization of the cDNA encoding rice elongation factor 1 beta.
作者信息
Matsumoto S, Terui Y, Xi S, Taira H, Ejiri S
机构信息
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka, Japan.
出版信息
FEBS Lett. 1994 Jan 24;338(1):103-6. doi: 10.1016/0014-5793(94)80125-8.
We have cloned and sequenced a cDNA coding for rice elongation factor 1 beta (EF-1 beta). The clone was 1420 bp long and contained an open reading frame coding for 229 amino acids. The overall identity between rice EF-1 beta and rice EF-1 beta' [Matsumoto, S., Oizumi, N., Taira, H. and Ejiri, S. (1992) FEBS Lett. 311, 46-48] is 60% at the amino acid sequence level; a higher percent identical residues (81%) were especially observed in the C-terminal region. Rice EF-1 beta has no conserved phosphorylation site for casein kinase II and no leucine zipper motif, although these motifs are well conserved in EF-1 delta (= beta in plants) subunits of animal EF-1.
我们已克隆并测序了编码水稻延伸因子1β（EF-1β）的cDNA。该克隆片段长1420 bp，包含一个编码229个氨基酸的开放阅读框。水稻EF-1β与水稻EF-1β'[松本，S.，大泉，N.，平良，H.和江尻，S.（1992年）《欧洲生物化学学会联合会快报》311，46 - 48]在氨基酸序列水平上的总体一致性为60%；在C端区域尤其观察到更高比例的相同残基（81%）。水稻EF-1β没有酪蛋白激酶II的保守磷酸化位点，也没有亮氨酸拉链基序，尽管这些基序在动物EF-1的EF-1δ（=植物中的β）亚基中保守性良好。
Zotero 插件