Kidou S, Ejiri S
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Japan.
Plant Mol Biol. 1998 Jan;36(1):137-48. doi: 10.1023/a:1005960721762.
Four different cDNA clones encoding protein synthesis elongation factor 1A, eEF1A, were isolated from rice (Oryza sativa L.). The genes encoded by these cDNAs were designated rice elongation factor 1A genes refa1, refa2, refa3 and refa4. The genes encoded identical eEF-1A polypeptides and shared high amino acid identity with eEF1A of other eukaryotes. Southern blot analysis suggested that some of these refa genes may be organized in a cluster on the same chromosome within a short distance. PCR analysis of rice genomic DNA showed that refa1 and refa4, and refa3 and refa2 are in neighboring locations on the rice genome. The mRNAs of the four refa genes accumulated to nearly equal levels in a variety of tissues and at different stages of growth. Suspension-cultured cells were the most abundant in refa mRNAs. Dormant seeds contained a small amount of the four refa mRNAs. Transcript accumulation was highly induced after seed germination, and the same expression levels were maintained even in old leaf blades of mature plants.
从水稻（Oryza sativa L.）中分离出了四个编码蛋白质合成延伸因子1A（eEF1A）的不同cDNA克隆。这些cDNA所编码的基因被命名为水稻延伸因子1A基因refa1、refa2、refa3和refa4。这些基因编码相同的eEF-1A多肽，并且与其他真核生物的eEF1A具有高度的氨基酸同一性。Southern杂交分析表明，其中一些refa基因可能在同一条染色体上短距离内成簇排列。水稻基因组DNA的PCR分析表明，refa1和refa4以及refa3和refa2在水稻基因组中相邻。这四个refa基因的mRNA在各种组织和不同生长阶段积累到几乎相等的水平。悬浮培养细胞中refa mRNA含量最高。休眠种子中含有少量的四种refa mRNA。种子萌发后转录物积累被高度诱导，即使在成熟植株的老叶片中也保持相同的表达水平。
