Fujita K, Nagatsu T, Shinpo K, Maruta K, Teradaira R, Nakamura M
Clin Chem. 1980 Oct;26(11):1577-82.
This method for assay of polyamines (putrescine, spermidine, and spermine) in large numbers of urine samples is based on preliminary analyte isolation by use of a Dowex 50W column, separation by high-voltage paper electrophoresis, reaction with ninhydrin to detect the separated amines, and subsequent direct assay with a dual-wave-length thin-layer chromatography scanner by the zigzag scanning method. By optimizing the conditions for the Dowex 50W column and high-voltage paper electrophoresis, putrescine, cadaverine, spermidine, and spermine were completely separated. The major technical improvements are room-temperature collection of urine with toluene, shorter hydrolysis time, complete separation of putrescine from cadaverine and histamine, and elimination of interference with spermine measurement. Polyamine concentrations as measured by this method agreed with those obtained by use of an amino acid analyzer. Reference intervals by this method were similar to those reported by Russell (Clin. Chem. 23: 22, 1977) with an automated amino acid analyzer. Putrescine, spermidine, and spermine excretion in urine from patients with blood cancers and solid cancers was significantly increased; information on concentrations of putrescine and spermidine were especially useful for following the clinical efficacy of cancer treatments.
这种用于检测大量尿液样本中多胺（腐胺、亚精胺和精胺）的方法基于以下步骤：首先使用Dowex 50W柱对分析物进行初步分离，然后通过高压纸电泳进行分离，与茚三酮反应以检测分离出的胺，随后使用双波长薄层色谱扫描仪通过锯齿扫描法进行直接检测。通过优化Dowex 50W柱和高压纸电泳的条件，腐胺、尸胺、亚精胺和精胺得以完全分离。主要技术改进包括用甲苯在室温下收集尿液、缩短水解时间、将腐胺与尸胺和组胺完全分离以及消除对精胺测量的干扰。用该方法测得的多胺浓度与使用氨基酸分析仪测得的结果一致。该方法的参考区间与Russell（《临床化学》23: 22, 1977）使用自动氨基酸分析仪报告的参考区间相似。血液癌症和实体癌症患者尿液中的腐胺、亚精胺和精胺排泄量显著增加；腐胺和亚精胺浓度信息对于跟踪癌症治疗的临床疗效特别有用。
