Seravalli Javier, Portugal Frank
Redox Biology Center and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Department of Biology, The Catholic University of America, Washington, DC 20064, USA.
Pathogens. 2023 Jun 27;12(7):881. doi: 10.3390/pathogens12070881.
Most forms of life, including the archaea, bacteria, and eukaryotes synthesize the polyamine putrescine. Although putrescine is widely distributed, several Gram-positive bacteria, including (), appear to be the exceptions. We report here that strains of can produce the polyamine putrescine, as well as the derivative -acetyl-putrescine. Three strains of from the American Type Culture Collection (ATCC), one strain listed in the National Center for Biotechnology Information (NCBI) database, whose genomic sequence is well defined, and well as eight strains from -induced brain abscesses of individual patients from multiple geographic locations were evaluated. Each strain was grown in complete chemically defined medium (CDM) under stringent conditions, after which the partially purified conditioned medium (CM) was analyzed by mass spectroscopy (MS), and the data were reported as the of experimental results to controls. We confirmed the synthesis of putrescine by by using C/N-labeled arginine as a tracer. We found that agmatine, -acetyl-putrescine, ornithine, citrulline, proline, and NH were all labeled with heavy isotope derived from C/N-labeled arginine. None of the strains examined produced spermine or spermidine, but strains from either ATCC or human brain abscesses produced putrescine and/or its derivative -acetyl-putrescine.
大多数生命形式，包括古细菌、细菌和真核生物，都会合成多胺腐胺。尽管腐胺分布广泛，但包括（）在内的几种革兰氏阳性菌似乎是例外。我们在此报告，（某菌）菌株能够产生多胺腐胺及其衍生物N-乙酰腐胺。对来自美国模式培养物保藏中心（ATCC）的三株（某菌）、一株列于国家生物技术信息中心（NCBI）数据库且基因组序列明确的（某菌），以及来自多个地理位置的个别患者因（某菌）引起的脑脓肿的八株（某菌）进行了评估。将每株菌在严格条件下于完全化学限定培养基（CDM）中培养，之后通过质谱（MS）分析部分纯化的条件培养基（CM），并将数据报告为实验结果与对照的比值。我们以C/N标记的精氨酸作为示踪剂，证实了（某菌）能合成腐胺。我们发现胍丁胺、N-乙酰腐胺、鸟氨酸、瓜氨酸、脯氨酸和NH均被源自C/N标记精氨酸的重同位素标记。所检测的菌株均未产生精胺或亚精胺，但来自ATCC或人脑脓肿的菌株产生了腐胺和/或其衍生物N-乙酰腐胺。
