腹股沟肉芽肿的血清学检测。
A serological test for granuloma inguinale.
作者信息
Freinkel A L, Dangor Y, Koornhof H J, Ballard R C
机构信息
Department of Anatomical Pathology, University of the Witwatersrand Medical School, Johannesburg, South Africa.
出版信息
Genitourin Med. 1992 Aug;68(4):269-72. doi: 10.1136/sti.68.4.269.
OBJECTIVES
An indirect immunofluorescence technique applied to paraffin embedded tissue sections of lesions containing Donovan bodies was evaluated as a serological test for the diagnosis of granuloma inguinale.
METHODS
Sera from patients with proven granuloma inguinale, other sexually acquired genital ulcerations and blood donors from areas where granuloma inguinale is rarely encountered as well as from disease-endemic regions were tested. Sera were tested either unabsorbed or following absorption with whole Klebsiella pneumoniae bacteria.
RESULTS
Using unabsorbed sera at a dilution of 1:160 the test was found to have a sensitivity of 100%, specificity of 98%, positive predictive value (PPV) of 89% and negative predictive value (NPV) of 100%. There proved to be no advantage in preabsorbing sera with K. pneumoniae antigen.
CONCLUSIONS
In the absence of culture methods for Calymmatobacterium granulomatis, an indirect immunofluorescence technique may prove valuable for the diagnosis of individual cases of granuloma inguinale and as an epidemiological tool in studies of the disease.
目的
评估一种应用于含杜诺凡小体病变石蜡包埋组织切片的间接免疫荧光技术，作为腹股沟肉芽肿诊断的血清学检测方法。
方法
检测确诊为腹股沟肉芽肿患者、其他性传播获得性生殖器溃疡患者的血清，以及来自腹股沟肉芽肿罕见地区和疾病流行地区的献血者血清。血清未吸收或用肺炎克雷伯菌全菌吸收后进行检测。
结果
使用未吸收血清，稀释度为1:160时，该检测方法的灵敏度为100%，特异性为98%，阳性预测值（PPV）为89%，阴性预测值（NPV）为100%。用肺炎克雷伯菌抗原预吸收血清未显示出优势。
结论
在缺乏肉芽肿荚膜杆菌培养方法的情况下，间接免疫荧光技术可能对个别腹股沟肉芽肿病例的诊断有价值，并可作为该疾病研究中的一种流行病学工具。
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