大肠杆菌中的阳离子转运。III. 稳态下的钾离子通量
Cation transport in Escherichia coli. III. Potassium fluxes in the steadystate.
作者信息
SCHULTZ S G, EPSTEIN W, GOLDSTEIN D A
出版信息
J Gen Physiol. 1962 Nov;46(2):343-53. doi: 10.1085/jgp.46.2.343.
Abstract
The present study is concerned with the measurement of the unidirectional K flux in E. coli. Methods are described by means of which a fairly dense suspension of cells may be maintained in a well defined steady-state with respect to the intracellular K concentration and the pH of the medium. The kinetics of K(42) exchange under these conditions are consistent with the presence of a single intracellular K compartment with a unidirectional K flux of 1 pmol/(cm(2) sec.). This rate is independent of the extracellular K concentration over the range studied. The simultaneous rate of H secretion averages 16 pmols/(cm(2) sec.) indicating that in the steady-state the efflux of metabolically produced H is not linked mole for mole to K movement.
摘要
本研究关注大肠杆菌中单向钾通量的测量。文中描述了一些方法，通过这些方法可以使细胞的相当密集的悬浮液在细胞内钾浓度和培养基pH值方面保持明确的稳态。在这些条件下钾（42）交换的动力学与单个细胞内钾区室的存在一致，其单向钾通量为1皮摩尔/（平方厘米·秒）。在所研究的范围内，该速率与细胞外钾浓度无关。同时，氢离子分泌的平均速率为16皮摩尔/（平方厘米·秒），这表明在稳态下，代谢产生的氢离子外流与钾的移动并非等摩尔关联。
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