Strugnell R, Dougan G, Chatfield S, Charles I, Fairweather N, Tite J, Li J L, Beesley J, Roberts M
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent, United Kingdom.
Infect Immun. 1992 Oct;60(10):3994-4002. doi: 10.1128/iai.60.10.3994-4002.1992.
The P.69 Bordetella pertussis protective antigen was expressed by use of the trc promoter from the chromosome of a Salmonella typhimurium aro vaccine strain, BRD509, by integrating the prn gene, encoding the 93-kDa precursor of this protein, into the aroC locus. P.69 was detected on the cell surface of the S. typhimurium strain (BRD640) by agglutination and immunoelectron microscopy. BALB/c mice immunized orally or intravenously with BRD640 showed a significant level of protection against an aerosol challenge with virulent B. pertussis, compared with control animals. No anti-P.69 antibodies in the serum or anti-P.69 antibody-secreting cells in the lungs were detected in BRD640-vaccinated animals, although cells isolated from spleens showed a P.69-dependent cell proliferative response. In contrast, low levels of anti-P.69 antibodies in the serum and anti-P.69 antibody-secreting cells in the lungs were detected in immunized mice following a B. pertussis challenge.
通过将编码该蛋白质93 kDa前体的prn基因整合到鼠伤寒沙门氏菌aro疫苗株BRD509染色体的trc启动子中，表达百日咳博德特氏菌P.69保护性抗原。通过凝集和免疫电子显微镜在鼠伤寒沙门氏菌菌株（BRD640）的细胞表面检测到P.69。与对照动物相比，经口服或静脉内免疫BRD640的BALB/c小鼠对强毒性百日咳博德特氏菌气溶胶攻击显示出显著水平的保护作用。在接种BRD640的动物中，未在血清中检测到抗P.69抗体，也未在肺中检测到分泌抗P.69抗体的细胞，尽管从脾脏分离的细胞显示出P.69依赖性细胞增殖反应。相比之下，在百日咳博德特氏菌攻击后的免疫小鼠中，在血清中检测到低水平的抗P.69抗体，在肺中检测到分泌抗P.69抗体的细胞。
