Grohmann U, Puccetti P, Romani L, Binaglia L, Bianchi R, Belladonna M L, Ullrich S J, Appella E, Fioretti M C
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Int J Cancer. 1992 Sep 30;52(3):372-7. doi: 10.1002/ijc.2910520308.
Highly immunogenic tumor variants are generated by in vitro or in vivo treatment of the murine L5178Y lymphoma line with triazene derivatives. Most of these variants express new transplantation- and antibody-defined antigens that previous studies have shown to be closely related. One such 80-kDa protein on the surface of clone-D cells was found to be related to xenotropic MuLV gp70 molecules. To investigate the possible relevance of clone-D data to general properties of immunogenic variants in this tumor model system, polyclonal syngeneic antisera raised to a panel of immunogenic clones (including clone D) of the drug-treated L5178Y lymphoma line were employed in the immunoprecipitation of cell-surface and intrinsically labeled variant cells. In all clones, 1- and 2-dimensional electrophoretic analysis of the immunoprecipitates detected an antigen of approximately 80 kDa, and 35S-labeled 80-kDa molecules could be cross-precipitated from all clones by the panel of clone-specific antisera. In addition, 45- and 30-kDa components were also found in metabolically labeled variant cells. While the surface 80-kDa component was reactive with anti-xenotropic gp70 antibodies, the 30-kDa molecule was removed by anti-gag p30 antibody in sequential immunoprecipitation experiments. These data suggest that expression of aberrant, retrovirus-related proteins is a common finding in immunogenic cells of the drug-treated L5178Y lymphoma line.
用三氮烯衍生物对小鼠L5178Y淋巴瘤细胞系进行体外或体内处理，可产生高免疫原性的肿瘤变体。这些变体中的大多数表达新的移植和抗体定义抗原，先前的研究表明它们密切相关。在克隆-D细胞表面发现的一种这样的80 kDa蛋白与嗜异源性MuLV gp70分子有关。为了研究克隆-D数据与该肿瘤模型系统中免疫原性变体的一般特性的可能相关性，将针对药物处理的L5178Y淋巴瘤细胞系的一组免疫原性克隆（包括克隆D）产生的多克隆同基因抗血清用于细胞表面和内在标记的变体细胞的免疫沉淀。在所有克隆中，对免疫沉淀物进行一维和二维电泳分析均检测到一种约80 kDa的抗原，并且克隆特异性抗血清组可从所有克隆中交叉沉淀出35S标记的80 kDa分子。此外，在代谢标记的变体细胞中还发现了45 kDa和30 kDa的成分。虽然表面80 kDa成分与抗嗜异源性gp70抗体反应，但在连续免疫沉淀实验中，30 kDa分子被抗gag p30抗体去除。这些数据表明，异常的、逆转录病毒相关蛋白的表达是药物处理的L5178Y淋巴瘤细胞系免疫原性细胞中的常见现象。
