8-叠氮基-ATP对牛心线粒体ATP酶的特异性光标记
Specific photolabelling of beef-heart mitochondrial ATPase by 8-azido-ATP.
作者信息
Wagenvoord R J, Van der Kraan I, Kemp A
出版信息
Biochim Biophys Acta. 1977 Apr 11;460(1):17-24. doi: 10.1016/0005-2728(77)90147-5.
PMID:139919
Abstract
- 8-Azido-ATP is a suitable photoaffinity label for beef-heart mitochondrial ATPase (F1) 2. 8-Azido-ATP is hydrolysed slowly by F1 in the dark. Photolysis at 350 nm in the presence of F1 leads to inhibition of the ATPase activity. The presence of ATP during illumination prevents the inhibition. Illumination of F1 in the absence of 8-azido-ATP causes no inhibition. 3. Added Mg2+ is not necessary for the binding of the 8-azido-ATP to F1. 4. 8-Azido-ATP binds specifically to the beta subunits of F1. 5. The ATPase activity is completely inhibited when 2 mol of 8-azido-ATP are bound per mol F1.
摘要
8-叠氮基-ATP是用于牛心线粒体ATP酶（F1）的合适光亲和标记物。
8-叠氮基-ATP在黑暗中被F1缓慢水解。在F1存在的情况下于350nm处进行光解会导致ATP酶活性受到抑制。光照期间ATP的存在可防止这种抑制作用。在没有8-叠氮基-ATP的情况下对F1进行光照不会产生抑制作用。
添加Mg2+对于8-叠氮基-ATP与F1的结合不是必需的。
8-叠氮基-ATP特异性结合到F1的β亚基上。
当每摩尔F1结合2摩尔8-叠氮基-ATP时，ATP酶活性被完全抑制。
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