WALLIS C, MELNICK J L
J Bacteriol. 1962 Sep;84(3):389-92. doi: 10.1128/jb.84.3.389-392.1962.
Wallis, Craig (Baylor University, Houston, Texas) and Joseph L. Melnick. Thermal inactivation of poliovirus under anaerobic conditions. J. Bacteriol. 84:389-392. 1962.-Of the six strains of poliovirus tested (three antigenic types), none was stabilized at 50 C under anaerobic conditions. The previously reported stabilization of polioviruses using thioglycolate had been attributed to anaerobic conditions. However, the sparing effect attained with high concentrations of thioglycolate was achieved as readily with other organic salts (benzoate, acetate) which could not be attributed to the anaerobic state. The viruses failed to be stabilized when low oxygen tension was obtained by dilute thioglycolate, l-cysteine, and nitrogen.
沃利斯，克雷格（贝勒大学，得克萨斯州休斯顿）和约瑟夫·L·梅尔尼克。厌氧条件下脊髓灰质炎病毒的热灭活。《细菌学杂志》84:389 - 392。1962年。——在所测试的6株脊髓灰质炎病毒（三种抗原型）中，没有一株在厌氧条件下于50℃时得到稳定。先前报道的使用巯基乙酸盐使脊髓灰质炎病毒稳定的情况曾被归因于厌氧条件。然而，高浓度巯基乙酸盐所达到的保护作用，使用其他有机盐（苯甲酸盐、乙酸盐）也同样容易实现，而这些有机盐不能归因于厌氧状态。当通过稀释的巯基乙酸盐、L - 半胱氨酸和氮气获得低氧张力时，病毒未能得到稳定。
