Thermal inactivation of poliovirus under anaerobic conditions.

Wallis, Craig (Baylor University, Houston, Texas) and Joseph L. Melnick. Thermal inactivation of poliovirus under anaerobic conditions. J. Bacteriol. 84:389-392. 1962.-Of the six strains of poliovirus tested (three antigenic types), none was stabilized at 50 C under anaerobic conditions. The previously reported stabilization of polioviruses using thioglycolate had been attributed to anaerobic conditions. However, the sparing effect attained with high concentrations of thioglycolate was achieved as readily with other organic salts (benzoate, acetate) which could not be attributed to the anaerobic state. The viruses failed to be stabilized when low oxygen tension was obtained by dilute thioglycolate, l-cysteine, and nitrogen.