高效液相色谱法测定血浆和尿液中布比卡因、2,6-哌啶二酰苯胺及4'-羟基布比卡因的含量。
High-performance liquid chromatographic method for the quantitation of bupivacaine, 2,6-pipecoloxylidide and 4'-hydroxybupivacaine in plasma and urine.
作者信息
Kastrissios H, Hung M F, Triggs E J
机构信息
Department of Pharmacy, University of Queensland, Australia.
出版信息
J Chromatogr. 1992 May 20;577(1):103-7. doi: 10.1016/0378-4347(92)80603-n.
A high-performance liquid chromatographic method with ultraviolet detection at 210 nm for quantitation of bupivacaine and two of its metabolites from plasma and urine is described. The compounds are extracted into n-hexane-isopropanol (5:1), evaporated and the reconstituted residue injected onto a reversed phase C18 column. Standard curves for all compounds were linear (r2 greater than 0.999) in the range 20-2000 ng/ml, with a limit of detection of 10 ng/ml. The inter-day coefficients of variation ranged between 2.7 and 12.2%. The method was applied to analyse bupivacaine and metabolite concentrations in patients on long-term epidural bupivacaine-fentanyl infusions.
本文描述了一种高效液相色谱法，用于定量检测血浆和尿液中的布比卡因及其两种代谢物，检测波长为210nm的紫外检测。化合物用正己烷-异丙醇（5:1）萃取，蒸发后将重构残渣注入反相C18柱。所有化合物的标准曲线在20-2000ng/ml范围内呈线性（r2大于0.999），检测限为10ng/ml。日内变异系数在2.7%至12.2%之间。该方法用于分析长期接受硬膜外布比卡因-芬太尼输注患者的布比卡因和代谢物浓度。
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