毛细管电泳法测定大鼠尿液中布比卡因及其三种代谢物
Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis.
作者信息
Schieferecke M A, McLaughlin K J, Faibushevich A A, Lunte C E
机构信息
Department of Chemistry, University of Kansas, Lawrence 66045, USA.
出版信息
Electrophoresis. 1998 Nov;19(16-17):2997-3002. doi: 10.1002/elps.1150191633.
A capillary electrophoretic (CE) method for the analysis of urinary extracts of the local anesthetic, bupivacaine, and its three main metabolites, desbutylbupivacaine, 3'-hydroxybupivacaine, and 4'-hydroxybupivacaine, in rat urine has been developed. The limits of detection were 0.22 microM for desbutylbupivacaine and bupivacaine, 0.15 microM for 3'-hydroxybupivacaine, and 0.16 microM for 4'-hydroxybupivacaine. The linear range was from 0.7 microM to 16.8 microM for all four compounds. Migration time and peak height reproducibilities, and extraction efficiencies were determined for all four compounds. Peak height reproducibilities (n = 5) for the overall method were improved through the use of prilocaine as an internal standard. Peak height reproducibilities were 5.6% RSD for desbutylbupivacaine and bupivacaine, and 9.9% RSD for 3'-hydroxybupivacaine and 4'-hydroxybupivacaine. Migration time reproducibilities (n = 5) were 2.4% for all compounds. Urine samples were collected from rats administered therapeutic doses of bupivacaine and extracted using a solid-phase extraction method (SPE). Separation of bupivacaine and its metabolites was achieved in 15 min.
已开发出一种毛细管电泳（CE）方法，用于分析大鼠尿液中局部麻醉剂布比卡因及其三种主要代谢物——去丁基布比卡因、3'-羟基布比卡因和4'-羟基布比卡因的尿液提取物。去丁基布比卡因和布比卡因的检测限为0.22微摩尔，3'-羟基布比卡因的检测限为0.15微摩尔，4'-羟基布比卡因的检测限为0.16微摩尔。所有四种化合物的线性范围为0.7微摩尔至16.8微摩尔。测定了所有四种化合物的迁移时间和峰高重现性以及提取效率。通过使用丙胺卡因作为内标，提高了整个方法的峰高重现性（n = 5）。去丁基布比卡因和布比卡因的峰高重现性相对标准偏差（RSD）为5.6%，3'-羟基布比卡因和4'-羟基布比卡因的峰高重现性相对标准偏差为9.9%。所有化合物的迁移时间重现性（n = 5）为2.4%。从给予治疗剂量布比卡因的大鼠收集尿液样本，并使用固相萃取法（SPE）进行萃取。布比卡因及其代谢物在15分钟内实现分离。
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