Nashabeh W, el Rassi Z
Department of Chemistry, Oklahoma State University, Stillwater 74078-0447.
J Chromatogr. 1992 Apr 10;596(2):251-64. doi: 10.1016/0021-9673(92)85014-k.
Enzymophoresis with coupled heterogeneous capillary enzyme reactor-capillary zone electrophoresis was developed and evaluated in the area of nucleic acids. Ribonuclease T1, hexokinase and adenosine deaminase were successfully immobilized on the inner walls of short fused-silica capillaries through glutaraldehyde attachment. These open-tubular capillary enzyme reactors were quite stable for a prolonged period of use under operation conditions normally used in capillary zone electrophoresis. The capillary enzyme reactors coupled in series with capillary zone electrophoresis served as peak locator on the electropherogram, improved the system selectivity, and facilitated the quantitative determination of the analytes with good accuracy. Also, they allowed the on-line digestion and mapping of minute amounts of transfer ribonucleic acids, and the simultaneous synthesis and separation of nanogram quantities of oligonucleotides.
开发了结合异质毛细管酶反应器-毛细管区带电泳的酶电泳法，并在核酸领域进行了评估。核糖核酸酶T1、己糖激酶和腺苷脱氨酶通过戊二醛附着成功固定在短熔硅毛细管内壁上。这些开管毛细管酶反应器在毛细管区带电泳通常使用的操作条件下长时间使用时相当稳定。与毛细管区带电泳串联的毛细管酶反应器在电泳图上用作峰定位器，提高了系统选择性，并有助于准确地定量测定分析物。此外，它们还允许对微量转移核糖核酸进行在线消化和图谱分析，以及同时合成和分离纳克量的寡核苷酸。
