Dolnik V, Liu J, Banks J F, Novotny M V, Bocek P
Department of Chemistry, Indiana University, Bloomington 47405.
J Chromatogr. 1989 Oct 20;480:321-30. doi: 10.1016/s0021-9673(01)84301-6.
The influence of various parameters affecting separation of oligonucleotides by capillary zone electrophoresis has been examined. The effects of pH, ionic strength, and various additives, including highly charged cations such as spermine, were studied. Using polycytidines as model compounds, it was demonstrated that pH in the range of 5-8 and ionic strength in the range of 20-200 mmol/l do not influence the separation of oligonucleotides substantially. However, with the addition of spermine to the background electrolyte, migration order was inverted as the effective mobilities of the larger oligonucleotides were greatly decreased. With the addition of spermine and sodium dodecyl sulfate, the separation+ of these model oligonucleotides was also significantly affected. The best separation of a homologous series of polycytidines was obtained with a background electrolyte containing 60 mmol/l histidine, 30 mmol/l glutamic acid, 50 mmol/l sodium dodecyl sulfate and 3 mmol/l spermine.
研究了影响毛细管区带电泳分离寡核苷酸的各种参数的影响。研究了pH值、离子强度以及包括精胺等高电荷阳离子在内的各种添加剂的影响。以聚胞苷为模型化合物，结果表明，pH值在5-8范围内和离子强度在20-200 mmol/l范围内对寡核苷酸的分离影响不大。然而，向背景电解质中添加精胺后，由于较大寡核苷酸的有效迁移率大大降低，迁移顺序发生了反转。添加精胺和十二烷基硫酸钠后，这些模型寡核苷酸的分离也受到显著影响。使用含有60 mmol/l组氨酸、30 mmol/l谷氨酸、50 mmol/l十二烷基硫酸钠和3 mmol/l精胺的背景电解质，可实现聚胞苷同源系列的最佳分离。
