Wang S K, Patton D L, Kuo C C
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195.
J Clin Microbiol. 1992 Oct;30(10):2551-4. doi: 10.1128/jcm.30.10.2551-2554.1992.
Ascorbic acid (vitamin C) is an essential nutrient for humans. It may also be needed by Chlamydia trachomatis, an intracellular bacterium. We investigated the effects of vitamin C on the growth of C. trachomatis E/UW-5/Cx in a primary culture of human amniotic epithelial cells. The results showed that vitamin C enhances C. trachomatis infection at concentrations of 0.2, 0.6, and 1.2 mg/dl (P less than 0.001). These three concentrations represent the in vivo concentrations of deficiency, normal, and overload levels in serum, respectively. The enhancement was dose dependent. However, the growth of C. trachomatis was inhibited at vitamin C concentrations of 120 and 1,200 mg/dl. The inhibitory effect of erythromycin against C. trachomatis was shown to be reduced in the presence of vitamin C at the three concentrations tested (P less than 0.025-0.001), and MICs were four times greater (1.6 versus 0.4 micrograms/ml). Human amniotic cells were tolerant to vitamin C concentrations of up to 1,200 mg/dl. The results show that vitamin C may be an important nutrient for C. trachomatis and that incorporation of vitamin C in the culture medium may enhance the isolation and propagation of C. trachomatis in cell cultures.
抗坏血酸（维生素C）是人类必需的营养素。沙眼衣原体这种细胞内细菌可能也需要它。我们在人羊膜上皮细胞原代培养物中研究了维生素C对沙眼衣原体E/UW-5/Cx生长的影响。结果表明，维生素C在浓度为0.2、0.6和1.2毫克/分升时可增强沙眼衣原体感染（P小于0.001）。这三个浓度分别代表血清中缺乏、正常和过载水平的体内浓度。这种增强呈剂量依赖性。然而，在维生素C浓度为120和1200毫克/分升时，沙眼衣原体的生长受到抑制。在所测试的三个浓度下，维生素C存在时，红霉素对沙眼衣原体的抑制作用降低（P小于0.025至0.001），最低抑菌浓度（MIC）增加四倍（1.6微克/毫升对0.4微克/毫升）。人羊膜细胞对高达1200毫克/分升的维生素C浓度具有耐受性。结果表明，维生素C可能是沙眼衣原体的一种重要营养素，在培养基中加入维生素C可能会增强沙眼衣原体在细胞培养物中的分离和繁殖。
