BADER J P, MORGAN H R
J Exp Med. 1961 Feb 1;113(2):271-81. doi: 10.1084/jem.113.2.271.
A study of the metabolic requirements for the growth of psittacosis virus in L cells has been extended to the water-soluble vitamins. In a system in which a balanced salt solution was used to deplete the cells of their vitamin constituents, only thiamine was essential for psittacosis virus production. Extended depletion of cells with media deficient in specific vitamins demonstrated that pantothenate, niacin (niacinamide), pyridoxine (pyridoxal), and choline, in addition to thiamine, were essential for maximal growth of psittacosis virus. No requirement for biotin, inositol, folic acid, or riboflavin was demonstrated, although the possibility of incomplete vitamin depletion of the cells has not been eliminated. In most cases in which a specific vitamin requirement was shown the decreased yield of virus was correlated with a delay in the cytopathic effects produced in the cell cultures by psittacosis virus.
一项关于鹦鹉热病毒在L细胞中生长的代谢需求的研究已扩展到水溶性维生素。在一个使用平衡盐溶液耗尽细胞维生素成分的系统中，只有硫胺素对鹦鹉热病毒的产生至关重要。用缺乏特定维生素的培养基对细胞进行长期耗尽实验表明，除硫胺素外，泛酸、烟酸（烟酰胺）、吡哆醇（吡哆醛）和胆碱对于鹦鹉热病毒的最大生长也是必不可少的。尽管尚未排除细胞维生素未完全耗尽的可能性，但未证明对生物素、肌醇、叶酸或核黄素存在需求。在大多数显示出对特定维生素有需求的情况下，病毒产量的降低与鹦鹉热病毒在细胞培养物中产生的细胞病变效应的延迟相关。
