Skakni L, Sardet A, Just J, Landman-Parker J, Costil J, Moniot-Ville N, Bricout F, Garbarg-Chenon A
Department of Microbiology and Molecular Biology, Centre Hospitalier Universitaire Saint Antoine, Paris, France.
J Clin Microbiol. 1992 Oct;30(10):2638-43. doi: 10.1128/jcm.30.10.2638-2643.1992.
The polymerase chain reaction (PCR) technique was used to detect Mycoplasma pneumoniae DNA in clinical samples (nasopharyngeal aspirations or bronchoalveolar lavages) obtained from 100 children, 1 month to 16 years old. PCR allowed the detection of M. pneumoniae DNA from 20 out of the 100 patients studied. In 16 cases, PCR positivity was associated with acute respiratory symptomatology. For five PCR-positive patients, a positive culture or a serological response evidenced acute M. pneumoniae infections. A lack of antibody response was observed particularly with immunocompromised children and infants less than 12 months old. The amount of M. pneumoniae DNA in the PCR was estimated in a semiquantitative way by comparison of its hybridization signal with those obtained for 100, 10, and 1 color-changing unit (CCU) of the M. pneumoniae FH strain. Small amounts (less than or equal to 10(2) CCU/ml) of M. pneumoniae were found in samples from asymptomatic patients, while larger amounts (greater than or equal to 10(2) to greater than or equal to 10(4) CCU/ml) were found for 8 out of 10 patients with acute pneumonia.
采用聚合酶链反应（PCR）技术检测了100名年龄在1个月至16岁儿童的临床样本（鼻咽抽吸物或支气管肺泡灌洗液）中的肺炎支原体DNA。PCR检测出100名研究患者中有20名存在肺炎支原体DNA。在16例中，PCR阳性与急性呼吸道症状相关。对于5名PCR阳性患者，阳性培养或血清学反应证实存在急性肺炎支原体感染。尤其在免疫功能低下的儿童和12个月以下婴儿中观察到缺乏抗体反应。通过将其杂交信号与肺炎支原体FH菌株100、10和1个颜色变化单位（CCU）所获得的信号进行比较，以半定量方式估计PCR中肺炎支原体DNA的量。在无症状患者的样本中发现少量（小于或等于10² CCU/ml）肺炎支原体，而在10例急性肺炎患者中有8例发现大量（大于或等于10²至大于或等于10⁴ CCU/ml）肺炎支原体。
