用于鉴定分枝杆菌的非放射性DNA探针的评估
Evaluation of nonradioactive DNA probes for identification of mycobacteria.
作者信息
Lebrun L, Espinasse F, Poveda J D, Vincent-Levy-Frebault V
机构信息
Service de Microbiologie, Hôpital Antoine Béclère, Clamart, France.
出版信息
J Clin Microbiol. 1992 Sep;30(9):2476-8. doi: 10.1128/jcm.30.9.2476-2478.1992.
Abstract
Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.
摘要
采用商业化学发光DNA探针（Accuprobe；基因探针公司，加利福尼亚州圣地亚哥）对结核分枝杆菌（MTB）复合群、鸟分枝杆菌复合群（MAC）、戈登分枝杆菌和堪萨斯分枝杆菌进行鉴定，并对134株临床分离株进行了评估。这些分离株包括36株MTB复合群、40株MAC、27株戈登分枝杆菌、9株堪萨斯分枝杆菌和22株分枝杆菌属。这四种探针的特异性均为100%。MTB复合群和戈登分枝杆菌探针的灵敏度为100%，MAC探针的灵敏度为95.2%。所检测的9株堪萨斯分枝杆菌中有5株未被该探针检测到。
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