Tortoli E, Simonetti M T, Lavinia F
Microbiological and Virological Laboratory, Careggi Hospital, Florence, Italy.
J Clin Microbiol. 1996 Nov;34(11):2838-40. doi: 10.1128/jcm.34.11.2838-2840.1996.
A panel of 104 isolates belonging to the species Mycobacterium kansasii and 78 mycobacterial isolates belonging to other species was tested in parallel with the present commercially available DNA probe (AccuProbe; Gen-Probe) and with a new probe just developed by the same manufacturer. While the old version of the probe confirmed the previously reported low sensitivity (only 59% of the M. kansasii isolates reacted), the new one was 100% sensitive. Only two non-M. kansasii strains, both M. gastri isolates, gave false-positive hybridization results.
一组104株堪萨斯分枝杆菌分离株和78株其他分枝杆菌分离株与目前市售的DNA探针(AccuProbe;Gen-Probe)以及同一制造商刚刚开发的一种新探针同时进行了检测。虽然旧版本的探针证实了之前报道的低灵敏度(只有59%的堪萨斯分枝杆菌分离株有反应),但新探针的灵敏度为100%。只有两株非堪萨斯分枝杆菌菌株,均为胃分枝杆菌分离株,出现了假阳性杂交结果。