Kazuyama Y
Research and Development Center of Hygienic Science, Kitasato University.
Kansenshogaku Zasshi. 1992 Feb;66(2):189-93. doi: 10.11150/kansenshogakuzasshi1970.66.189.
The author devised a method which permits simultaneous isolation and identification of Chlamydia using a slide chamber with 8 wells. Contaminating bacteria were eliminated by filtration with the membrane filter. The procedure allowed isolation of Chlamydia pneumoniae and Chlamydia trachomatis from 11 (10.1%) out of 109 and 10 (9.2%) out of 109 otolaryngologic clinical specimens, respectively. The use of HL cells together with Hela-229 cells had been considered essential to isolate Chlamydia from specimens in this field. The conditions for isolating Chlamydia pneumoniae were similar to those for Chlamydia trachomatis. The slide chamber procedure with membrane filter treated specimens was suitable for simultaneous isolation and culture of various species of Chlamydia and allowed easy differentiation of Chlamydia pneumoniae from Chlamydia trachomatis with identification by the indirect fluorescent-antibody technique. Routine examination of clinical specimens using this procedure should be required in the future in order to better understand chlamydial infections.
作者设计了一种方法，该方法允许使用带有8个孔的载玻片腔室同时分离和鉴定衣原体。通过用膜过滤器过滤来消除污染细菌。该程序分别从109份耳鼻喉科临床标本中的11份（10.1%）和109份中的10份（9.2%）中分离出肺炎衣原体和沙眼衣原体。在该领域，人们认为将HL细胞与Hela - 229细胞一起使用对于从标本中分离衣原体至关重要。分离肺炎衣原体的条件与沙眼衣原体相似。用膜过滤器处理标本的载玻片腔室程序适用于同时分离和培养各种衣原体，并通过间接荧光抗体技术鉴定，从而便于区分肺炎衣原体和沙眼衣原体。为了更好地了解衣原体感染，未来应要求使用该程序对临床标本进行常规检查。
