CLARK W A
Appl Microbiol. 1962 Sep;10(5):466-71. doi: 10.1128/am.10.5.466-471.1962.
A wide variety of bacteriophages were processed and stored under different conditions to compare methods for long-term preservation. Specimens were stored for 2 years at room temperature (24 to 28 C) and at 4 C as broth lysates in 50% glycerol, dried, and freeze-dried. Titers determined after processing indicated that, of the broth, glycerol, and freeze-dry methods, freeze-drying was most damaging to the phages tested, glycerol less damaging, and the broth method least damaging. After 2 years, titers of broth lysates were generally higher than those of glycerol or freeze-dried preparations. Dried preparations generally did not prove satisfactory. Preparations stored at 4 C showed better titers than those kept at room temperature. All titers declined with time regardless of the conditions of preservation.
处理了各种各样的噬菌体，并将它们保存在不同条件下，以比较长期保存的方法。样本在室温（24至28摄氏度）和4摄氏度下作为50%甘油肉汤裂解物保存2年，进行干燥和冻干处理。处理后测定的效价表明，在肉汤、甘油和冻干方法中，冻干对所测试的噬菌体损害最大，甘油损害较小，肉汤法损害最小。2年后，肉汤裂解物的效价通常高于甘油或冻干制剂。干燥制剂总体上并不令人满意。保存在4摄氏度的制剂比保存在室温下的制剂效价更高。无论保存条件如何，所有效价都随时间下降。
