一种噬菌体微凝胶可有效治疗烧伤创面的多重耐药细菌感染。

A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Bacterial Infections in Burn Wounds.

作者信息

Dehari Deepa, Chaudhuri Aiswarya, Kumar Dulla Naveen, Patil Rohit, Gangwar Mayank, Rastogi Sonam, Kumar Dinesh, Nath Gopal, Agrawal Ashish Kumar

机构信息

Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India.

Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India.

出版信息

Pharmaceuticals (Basel). 2023 Jun 29;16(7):942. doi: 10.3390/ph16070942.

Multidrug-resistant (MDR) () is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the -specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR clinical strains. Isolated BPABΦ1 were found to belong to the family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application.

多重耐药（MDR）（）是烧伤创面感染中存在的主要病原体之一。生物膜的形成使得使用临床可用抗生素治疗变得更具挑战性。在当前的工作中，我们分离出了特异性噬菌体（BPABΦ1），将其载入壳聚糖微粒，随后分散在凝胶中，并评估了其对MDR临床菌株的治疗效果。发现分离出的BPABΦ1属于科，每个受感染宿主细胞的裂解量为102.12±2.65个噬菌斑形成单位。对载入BPABΦ1的壳聚糖微粒进行了质量属性评估，即尺寸、多分散指数、表面形态、体外释放等。所开发的制剂在体外表现出优异的抗生物膜根除潜力，局部应用后能有效促进伤口愈合。