百日咳诊断中的培养与荧光抗体法
CULTURE AND FLUORESCENT-ANTIBODY METHODS IN DIAGNOSIS OF WHOOPING COUGH.
作者信息
HOLWERDA J, ELDERING G
出版信息
J Bacteriol. 1963 Sep;86(3):449-51. doi: 10.1128/jb.86.3.449-451.1963.
Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining procedures applied to direct slide preparations. A total of 138 were positive by both methods, 25 by culture only, and 25 by FA only. The FA technique was also used in the identification of young cultures. It was shown that a positive culture report could be speeded up by about 1 day by this method. Without FA, onehalf the positive culture reports were made in 3 days; with FA, 75% were reported in a similar period.
霍尔韦尔达，杰克（密歇根州卫生部，大急流城）和格蕾丝·埃尔德林。百日咳诊断中的培养法和荧光抗体法。《细菌学杂志》86:449 - 451。1963年。——对517名疑似百日咳患者的鼻咽拭子进行了培养检查，并对直接涂片制剂应用荧光抗体（FA）染色程序进行检查。两种方法均呈阳性的有138例，仅培养阳性的有25例，仅FA阳性的有25例。FA技术还用于鉴定早期培养物。结果表明，通过这种方法，阳性培养报告可加快约1天。没有FA时，一半的阳性培养报告在3天内做出；有FA时，75%在类似时间段内报告。
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