ELDERING G, EVELAND W C, KENDRICK P L
J Bacteriol. 1962 Apr;83(4):745-9. doi: 10.1128/jb.83.4.745-749.1962.
Eldering, Grace (Michigan Department of Health, Grand Rapids), Warren C. Eveland, and Pearl L. Kendrick. Fluorescent antibody staining and agglutination reactions in Bordetella pertussis cultures. J. Bacteriol. 83:745-749. 1962-Bordetella pertussis antisera produced with smooth cultures gave positive results in agglutination and fluorescent antibody (FA) tests with smooth B. pertussis cultures. Similar results were obtained with antisera produced with the related species B. parapertussis and B. bronchiseptica when each was tested with the respective homologous antigen. Cross reactions occurred with some of the antisera and heterologous antigens but a positive agglutination test was not always correlated with a positive FA reaction, and the reverse was also observed. Adsorbed B. pertussis antisera specific for factors 2, 3, 4, and 5 agglutinated appropriate B. pertussis cultures, but gave negative FA reactions. Factor 1 antiserum prepared by adsorption with heated (100 C) antigen gave positive results in both FA and agglutination tests. Rough B. pertussis cultures were inagglutinable, but were FA positive when tested with unadsorbed antiserum produced with either smooth or rough cultures. Rough cultures were FA negative with factor 1 serum. The results suggest that for B. pertussis different serum components may be responsible for agglutination and FA staining. The implications with respect to the protection-inducing antigen are discussed.
埃尔德林，格雷斯（密歇根州卫生部，大急流城）、沃伦·C·埃夫兰德和珀尔·L·肯德里克。百日咳博德特氏菌培养物中的荧光抗体染色和凝集反应。《细菌学杂志》83:745 - 749。1962年——用光滑型培养物制备的百日咳博德特氏菌抗血清在与光滑型百日咳博德特氏菌培养物进行凝集和荧光抗体（FA）试验时呈阳性结果。当用相关物种副百日咳博德特氏菌和支气管败血博德特氏菌的抗血清分别与各自的同源抗原进行检测时，也得到了类似结果。一些抗血清与异源抗原发生交叉反应，但阳性凝集试验并不总是与阳性FA反应相关，反之亦然。吸附的针对因子2、3、4和5的百日咳博德特氏菌抗血清能凝集合适的百日咳博德特氏菌培养物，但FA反应呈阴性。用加热（100℃）抗原吸附制备的因子1抗血清在FA和凝集试验中均呈阳性结果。粗糙型百日咳博德特氏菌培养物不能被凝集，但用光滑型或粗糙型培养物制备的未吸附抗血清检测时FA呈阳性。粗糙型培养物用因子1血清检测时FA呈阴性。结果表明，对于百日咳博德特氏菌，不同的血清成分可能负责凝集和FA染色。讨论了与诱导保护的抗原相关的意义。
