KINASES OF STREPTOCOCCUS FAECALIS. I. ENZYME LOCALIZATION.

Moore, L. Daneo (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Kinases of Streptococcus faecalis. I. Enzyme localization. J. Bacteriol. 86:766-772. 1963.-Cell-free extracts of Streptococcus faecalis 10C1 were found to contain a membrane-associated, particulate hexokinase with activity toward glucose, fructose, mannose, and, to a lesser extent, galactose, gluconate, and mannitol. In addition, the cell cytoplasm contained kinases specific for fructose, mannose, and galactose. No specific kinases were found for gluconate or mannitol, although in the presence of mannitol adenosine triphosphate was utilized by a cytoplasmic fraction. The possibility is discussed that the main pathways of gluconate and mannitol metabolism are oxidation followed by phosphorylation.