鼠伤寒沙门氏菌碳水化合物转运缺陷型突变体的遗传分析。
Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium.
作者信息
Levinthal M, Simoni R D
出版信息
J Bacteriol. 1969 Jan;97(1):250-5. doi: 10.1128/jb.97.1.250-255.1969.
Abstract
Mutants (car) isolated from Salmonella typhimurium were unable to utilize or ferment the following carbohydrates (all d-configuration): glucose, fructose, mannose, N-acetylglucosamine, sorbitol, mannitol, maltose, melibiose, and glycerol. The mutants did utilize galactose, glucose 6-phosphate, gluconic acid, glucuronic acid, pyruvate, and l-lactate. Biochemical analysis showed that there were two classes of mutants, each lacking one component of a phosphotransferase system. CarA mutants were deficient in enzyme I; carB lacked the phosphate carrier protein, HPr. Mapping experiments showed that the carA gene was located near pro; the carB gene mapped near purC.
摘要
从鼠伤寒沙门氏菌中分离出的突变体（car）无法利用或发酵以下碳水化合物（均为d-构型）：葡萄糖、果糖、甘露糖、N-乙酰葡糖胺、山梨醇、甘露醇、麦芽糖、蜜二糖和甘油。这些突变体能够利用半乳糖、6-磷酸葡萄糖、葡萄糖酸、葡糖醛酸、丙酮酸和L-乳酸。生化分析表明存在两类突变体，每类都缺少磷酸转移酶系统的一个组分。CarA突变体缺乏酶I；carB缺少磷酸载体蛋白HPr。定位实验表明carA基因位于pro附近；carB基因定位于purC附近。
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