NETER E, BERTRAM L F, ZAK D A, MURDOCK M R, ARBESMAN C E
J Exp Med. 1952 Jul;96(1):1-15. doi: 10.1084/jem.96.1.1.
A study on hemagglutination and hemolysis by Escherichia coli O111 and O55 (rabbit) antisera and on hemagglutination and hemolysis inhibition by E. coli O111 and O55 antigens revealed the following facts. 1. Red blood cells of man, dog, rabbit, guinea pig, sheep, rat, and chicken adsorb E. coli O111 and O55 antigens and thus become specifically agglutinable by the homologous E. coli antisera. 2. The adsorption of these E. coli antigens is a function of the concentration of the antigen, the time (from 5 minutes to 2 hours) of treatment of the red blood cells with the antigen, and the concentration of the red blood cells used. 3. Red blood cells of man and sheep adsorb simultaneously both antigens, as indicated by the fact that both antisera give agglutination of all red blood cells. Complete agglutination does not occur when a mixture of red blood cells treated separately with the two antigens is added to one or the other of the two antisera. 4. Treatment of red blood cells of man with one of the antigens does not block the adsorption of the second antigen. Human cells treated with either or both antigens are still agglutinated by the homologous blood group (A, B, and Rh)-specific antibodies. 5. In the presence of guinea pig complement, E. coli O111 and O55 antisera produce hemolysis of modified human red blood cells in titers of the same order of magnitude as those giving hemagglutination and bacterial agglutination. The same antisera produce hemolysis of sheep cells treated with the identical antigens in titers exceeding by far those giving agglutination of modified human or sheep red blood cells. 6. Both sediment and supernate of a boiled E. coli suspension are capable of modifying red blood cells for E. coli hemagglutination; in contrast, the supernate obtained from an unboiled suspension and then heated does not modify red blood cells for hemagglutination, although it contains the antigen which can specifically adsorb E. coli antibodies, as shown by means of the hemagglutination and hemolysis inhibition tests. 7. Both the unheated and the boiled suspensions of E. coli O111 and O55 inhibit hemagglutination and hemolysis specifically. 8. Rabbit red blood cells modified by either E. coli O111 or 055 antigens, upon intravenous injection into rabbits, engender specific E. coli antibodies. The significance of the results is discussed.
一项关于大肠杆菌O111和O55（兔）抗血清的血凝和溶血以及大肠杆菌O111和O55抗原的血凝和溶血抑制的研究揭示了以下事实。1. 人、狗、兔、豚鼠、绵羊、大鼠和鸡的红细胞吸附大肠杆菌O111和O55抗原，因此可被同源大肠杆菌抗血清特异性凝集。2. 这些大肠杆菌抗原的吸附是抗原浓度、红细胞与抗原处理时间（5分钟至2小时）以及所用红细胞浓度的函数。3. 人和绵羊的红细胞同时吸附两种抗原，这一事实表明两种抗血清都能使所有红细胞凝集。当将分别用两种抗原处理的红细胞混合物加入两种抗血清中的一种时，不会发生完全凝集。4. 用人红细胞用其中一种抗原处理不会阻止第二种抗原的吸附。用一种或两种抗原处理的人细胞仍可被同源血型（A、B和Rh）特异性抗体凝集。5. 在豚鼠补体存在下，大肠杆菌O111和O55抗血清对经修饰的人红细胞产生溶血，其效价与产生血凝和细菌凝集的效价处于同一数量级。相同的抗血清对用相同抗原处理的绵羊细胞产生的溶血效价远远超过对经修饰的人或绵羊红细胞产生凝集的效价。6. 煮沸的大肠杆菌悬液的沉淀物和上清液都能够使红细胞发生改变以用于大肠杆菌血凝；相反，从未煮沸的悬液中获得然后加热的上清液不会使红细胞发生改变以用于血凝，尽管它含有能够特异性吸附大肠杆菌抗体的抗原，这通过血凝和溶血抑制试验得以证明。7. 大肠杆菌O111和O55的未加热和煮沸悬液都能特异性抑制血凝和溶血。8. 用大肠杆菌O111或O55抗原修饰的兔红细胞静脉注射到兔体内后会产生特异性大肠杆菌抗体。讨论了结果的意义。
