During M J, Bean A J, Roth R H
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510.
Neurosci Lett. 1992 Jun 8;140(1):129-33. doi: 10.1016/0304-3940(92)90698-7.
The effect of CNS stimulant drugs on the in vivo release of the colocalized neurotransmitters dopamine and neurotensin in rat prefrontal cortex was studied using microdialysis. Amphetamine, methylphenidate and nomifensine all increased extracellular fluid (ECF) levels of dopamine; however, their effects of neurotensin varied. Amphetamine increased both ECF dopamine (514 +/- 82% of basal) and neurotensin (350 +/- 49% of basal); however, the neurotensin increase lagged behind the increase in dopamine suggesting a possible trans-synaptic effect. Methylphenidate increased both dopamine and neurotensin (226 +/- 26% and 151 +/- 14% of basal respectively) co-synchronously, suggesting exocytosis of vesicles containing both dopamine and neurotensin. The nomifensine-induced increase in dopamine (202 +/- 23% of basal) was similar to that of methylphenidate, whereas the increase in neurotensin was significantly delayed and of lower magnitude (134 +/- 20% of basal). These data suggest that dopamine and neurotensin in part share a common releaseable pool in the prefrontal cortex. Moreover, dopamine may act presynaptically to increase neurotensin release and the different behavioral profiles of these psychostimulants may in part relate to their different effects on neurotensin release.
采用微透析技术研究了中枢神经系统兴奋剂对大鼠前额叶皮质中共定位神经递质多巴胺和神经降压素体内释放的影响。苯丙胺、哌甲酯和诺米芬辛均增加了多巴胺的细胞外液（ECF）水平；然而，它们对神经降压素的影响各不相同。苯丙胺增加了ECF多巴胺（基础值的514±82%）和神经降压素（基础值的350±49%）；然而，神经降压素的增加滞后于多巴胺的增加，提示可能存在跨突触效应。哌甲酯同步增加了多巴胺和神经降压素（分别为基础值的226±26%和151±14%），提示含有多巴胺和神经降压素的囊泡发生了胞吐作用。诺米芬辛引起的多巴胺增加（基础值的202±23%）与哌甲酯相似，而神经降压素的增加明显延迟且幅度较小（基础值的134±20%）。这些数据表明，多巴胺和神经降压素在前额叶皮质部分共享一个共同的可释放池。此外，多巴胺可能通过突触前作用增加神经降压素的释放，这些精神兴奋剂不同的行为表现可能部分与其对神经降压素释放的不同影响有关。
