Yamasaki Y, Suzuki T, Yamaya H, Matsuura N, Onodera H, Kogure K
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Neurosci Lett. 1992 Aug 3;142(1):45-7. doi: 10.1016/0304-3940(92)90616-f.
To determine the contribution of interleukin 1 (IL-1) on ischemic brain edema formation, the effect of recombinant human interleukin 1 beta (rhIL-1 beta), or zinc protoporphyrin (ZnPP) as an IL-1 blocker, on brain edema was studied in rats. The animals were subjected to 60 min of ischemia in a middle cerebral artery occlusion model. Immediately after reperfusion, rhIL-1 beta at a dose of 10 ng/2 microliters, or ZnPP at doses of 1 and 10 micrograms/2 microliters were topically applied into lateral cerebroventricle. In rhIL-1 beta-treated rats, ischemic brain edema formation was significantly increased in the dorsal and ventral areas of the caudate putamen 24 h after reperfusion, compared to that of vehicle-treated control rats. Furthermore, in ZnPP-treated rats, brain edema was decreased in both caudate-putamen areas. This suggests that IL-1 plays an important role in pathogenesis for post-ischemic brain edema.
为了确定白细胞介素1（IL-1）对缺血性脑水肿形成的作用，研究了重组人白细胞介素1β（rhIL-1β）或作为IL-1阻滞剂的锌原卟啉（ZnPP）对大鼠脑水肿的影响。在大脑中动脉闭塞模型中，使动物经历60分钟的缺血。再灌注后立即将剂量为10 ng/2微升的rhIL-1β或剂量为1和10微克/2微升的ZnPP局部注入侧脑室。与用赋形剂处理的对照大鼠相比，在rhIL-1β处理的大鼠中，再灌注24小时后尾状核壳核的背侧和腹侧区域缺血性脑水肿形成显著增加。此外，在ZnPP处理的大鼠中，两个尾状核壳核区域的脑水肿均减少。这表明IL-1在缺血后脑水肿的发病机制中起重要作用。
