GLAUERT A M, KERRIDGE D, HORNE R W
J Cell Biol. 1963 Aug;18(2):327-36. doi: 10.1083/jcb.18.2.327.
The sheathed flagellum of Vibrio metchnikovii was chosen for a study of the attachment of the flagellum to the bacterial cell. Normal and autolysed organisms and isolated flagella were studied by electron microscopy using the techniques of thin sectioning and negative staining. The sheath of the flagellum has the same layered structure as the cell wall of the bacterium, and in favourable thin sections it appears that the sheath is a continuation of the cell wall. After autolysis the sheath is usually absent and the core of the flagellum has a diameter of 120 A. Electron micrographs of autolysed bacteria negatively stained with potassium phosphotungstate show that the core ends in a basal disc just inside the plasma membrane. The basal disc is about 350 A in diameter and is thus considerably smaller than the "basal granules" described previously by other workers.
梅契尼科夫弧菌的鞘鞭毛被选来研究鞭毛与细菌细胞的附着情况。通过超薄切片和负染色技术，利用电子显微镜对正常和自溶的生物体以及分离出的鞭毛进行了研究。鞭毛的鞘与细菌的细胞壁具有相同的分层结构，在合适的超薄切片中，鞘似乎是细胞壁的延续。自溶后，鞘通常消失，鞭毛的核心直径为120埃。用磷钨酸钾负染色的自溶细菌的电子显微照片显示，核心在质膜内侧的一个基盘处终止。基盘直径约为350埃，因此比其他研究者先前描述的“基粒”小得多。
