Charteris D G, Hiscott P, Grierson I, Lightman S L
Department of Clinical Science, Institute of Ophthalmology, London, United Kingdom.
Ophthalmology. 1992 Sep;99(9):1364-7. doi: 10.1016/s0161-6420(92)31793-2.
To investigate the potential contribution of inflammatory and immune-mediated processes contributing to the pathogenesis of proliferative vitreoretinopathy (PVR), an immunohistochemical study was undertaken to characterize the infiltrating inflammatory cells in epiretinal membranes surgically removed from the eyes of patients with PVR.
Twenty-one epiretinal membranes obtained surgically from eyes with PVR complicating rhegmatogenous retinal detachment were studied immunohistochemically using the ABC technique and a panel of monoclonal and polyclonal antibodies.
T lymphocytes were found in 18 of the 21 specimens and generally constituted a small percentage of the total cell number. CD4+ T cells were found in 14 of the 18 membranes containing T cells. Three of six frozen membranes contained T cells that were positive for the interleukin-2 receptor. In 5 of 16 membranes studied, cells positive for the macrophage/monocyte marker were found. No B lymphocytes or neutrophils were identified, and there were no deposits of complement or immunoglobulins. Positive staining for the class II MHC antigen HLA-DR was found in 7 of the 21 membranes, a result that was more consistent in frozen than in fixed tissues.
The study suggests that T lymphocytes are present in PVR epiretinal membranes and may be activated. These cells have the potential to play a role in the pathobiology of PVR.
为了研究炎症和免疫介导过程在增殖性玻璃体视网膜病变（PVR）发病机制中的潜在作用，进行了一项免疫组织化学研究，以鉴定从PVR患者眼中手术切除的视网膜前膜中浸润的炎症细胞特征。
采用ABC技术及一组单克隆和多克隆抗体，对21例因孔源性视网膜脱离并发PVR而手术获得的视网膜前膜进行免疫组织化学研究。
21个标本中有18个发现T淋巴细胞，且通常占细胞总数的比例较小。在18个含T细胞的膜中有14个发现CD4 + T细胞。6个冷冻膜中有3个含有白细胞介素-2受体阳性的T细胞。在16个研究的膜中有5个发现巨噬细胞/单核细胞标志物阳性的细胞。未发现B淋巴细胞或中性粒细胞，也没有补体或免疫球蛋白沉积。21个膜中有7个发现II类MHC抗原HLA-DR阳性染色，冷冻组织中的结果比固定组织中的更一致。
该研究表明T淋巴细胞存在于PVR视网膜前膜中且可能被激活。这些细胞有可能在PVR的病理生物学中发挥作用。
