Charteris D G, Hiscott P, Robey H L, Gregor Z J, Lightman S L, Grierson I
Department of Clinical Science, Institute of Ophthalmology, London, United Kingdom.
Ophthalmology. 1993 Jan;100(1):43-6. doi: 10.1016/s0161-6420(93)31711-2.
The inflammatory cell content of subretinal membranes occurring in proliferative vitreoretinopathy (PVR) has not yet been well characterized. This study was undertaken to investigate the inflammatory cells present in PVR subretinal membranes.
Eight subretinal membranes obtained surgically from eyes with PVR complicating rhegmatogenous retinal detachment were studied immunohistochemically using the avidin-biotin complex technique and a panel of monoclonal and polyclonal antibodies.
T lymphocytes were found in five of the eight subretinal membranes. CD4+ T cells were demonstrated in four and CD8+ T cells in one of the membranes. T cells bearing the interleukin 2 receptor were found in two of four membranes studied. Macrophages were found in four membranes. No B lymphocytes or neutrophils were demonstrated, and there were no significant deposits of complement or immunoglobulins. Expression of the MHC class II antigen HLA DR was consistently found in frozen specimens. Glial cells and retinal pigment epithelial cells also were present in the membranes, often adjacent to T cells, although overall no obvious relationship was found between glial or retinal epithelial cells and T cells.
The findings indicate that T lymphocytes are present in PVR subretinal membranes and have the potential to interact with other cell types in the pathogenesis of this condition.
增殖性玻璃体视网膜病变（PVR）中视网膜下膜的炎性细胞成分尚未得到充分表征。本研究旨在调查PVR视网膜下膜中存在的炎性细胞。
使用抗生物素蛋白-生物素复合物技术以及一组单克隆和多克隆抗体，对8例因PVR并发孔源性视网膜脱离而手术获取的视网膜下膜进行免疫组织化学研究。
在8例视网膜下膜中的5例发现了T淋巴细胞。在其中4例中证实有CD4 + T细胞，1例中有CD8 + T细胞。在所研究的4例膜中的2例发现了携带白细胞介素2受体的T细胞。在4例膜中发现了巨噬细胞。未证实有B淋巴细胞或中性粒细胞，也没有补体或免疫球蛋白的显著沉积。在冷冻标本中始终发现MHC II类抗原HLA DR的表达。膜中也存在神经胶质细胞和视网膜色素上皮细胞，它们通常与T细胞相邻，尽管总体上在神经胶质细胞或视网膜上皮细胞与T细胞之间未发现明显关系。
这些发现表明T淋巴细胞存在于PVR视网膜下膜中，并在这种疾病的发病机制中具有与其他细胞类型相互作用的潜力。
