粗糙脉孢菌的不饱和脂肪酸突变体。
Unsaturated fatty acid mutants of Neurospora crassa.
作者信息
Scott W A
出版信息
J Bacteriol. 1977 Jun;130(3):1144-8. doi: 10.1128/jb.130.3.1144-1148.1977.
Abstract
Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group V. Unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond position as well as different steric configurations were tested for growth requirements.
摘要
用N-甲基-N'-硝基-N-亚硝基胍处理粗糙脉孢菌的分生孢子，然后在含有悬浮于聚山梨醇酯NP-40中的多不饱和脂肪酸的培养基上进行分离，从而获得了不饱和脂肪酸（ufa）营养缺陷型。从与野生型杂交中重新分离得到的24个突变体被分为两个互补类，ufa-1和ufa-2，它们位于连锁群V上。测试了具有不同程度不饱和度、链长、双键位置以及不同空间构型的不饱和脂肪酸的生长需求。
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