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J Bacteriol. 1977 Jun;130(3):1144-8. doi: 10.1128/jb.130.3.1144-1148.1977.
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Fatty acid biosynthesis in novel ufa mutants of Neurospora crassa.粗糙脉孢菌新型不饱和脂肪酸突变体中的脂肪酸生物合成
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Isolation of circadian clock mutants of Neurospora crassa.粗糙脉孢菌生物钟突变体的分离。
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5
Complementation among cytoplasmic mutants of Neurospora crassa.粗糙脉孢菌细胞质突变体间的互补作用。
Mol Gen Genet. 1972;117(1):82-90. doi: 10.1007/BF00268840.
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Genetical and biochemical evidence for further interrelationships between the polyaromatic synthetic and the quinate-shikimate catabolic pathways in Neurospora crassa.粗糙脉孢菌中多芳香族合成途径与奎尼酸-莽草酸分解代谢途径之间进一步相互关系的遗传学和生化证据。
Genetics. 1972 Jul;71(3):337-48. doi: 10.1093/genetics/71.3.337.
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Endogenous purine metabolism in the conidia of wild type and certain adenine mutants of Neurospora crassa. I. The nature of the reserve pools and pool utilization during adenine starvation.粗糙脉孢菌野生型及某些腺嘌呤突变体分生孢子中的内源性嘌呤代谢。I. 腺嘌呤饥饿期间储备库的性质及库的利用情况。
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Characterization of Neurospora crassa mutants deficient in glucosephosphate isomerase.磷酸葡萄糖异构酶缺陷型粗糙脉孢菌突变体的特性分析。
J Bacteriol. 1975 Apr;122(1):54-8. doi: 10.1128/jb.122.1.54-58.1975.
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Circadian rhythms in Neurospora crassa: effects of unsaturated fatty acids.粗糙脉孢菌中的昼夜节律：不饱和脂肪酸的影响。
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Isolation and classification of extranuclear mutants of Neurospora crassa.粗糙脉孢菌核外突变体的分离与分类
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The WW domain protein PRO40 is required for fungal fertility and associates with Woronin bodies.WW结构域蛋白PRO40是真菌育性所必需的，并与沃罗宁体相关联。
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2
Metabolism of ricinoleate by Neurospora crassa.粗糙脉孢菌对蓖麻醇酸的代谢
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Chromosomal loci of Neurospora crassa.粗糙脉孢菌的染色体位点。
Microbiol Rev. 1982 Dec;46(4):426-570. doi: 10.1128/mr.46.4.426-570.1982.

本文引用的文献

1
Studies on a Neurospora mutant requiring unsaturated fatty acids for growth.关于一种生长需要不饱和脂肪酸的粗糙脉孢菌突变体的研究。
J Bacteriol. 1949 Nov;58(5):595-9. doi: 10.1128/jb.58.5.595-599.1949.
2
Efficient method for selection of auxotrophic mutants of Neurospora.用于选择粗糙脉孢菌营养缺陷型突变体的高效方法。
Science. 1959 Feb 27;129(3348):572. doi: 10.1126/science.129.3348.572.
3
Isolation of nutritional mutants of Neurospora crassa by filtration enrichment.通过过滤富集法分离粗糙脉孢菌的营养突变体。
J Gen Microbiol. 1954 Aug;11(1):34-6. doi: 10.1099/00221287-11-1-34.
4
Unsaturated fatty acid mutants of Saccharomyces cerevisiae.酿酒酵母的不饱和脂肪酸突变体。
J Bacteriol. 1966 Sep;92(3):597-600. doi: 10.1128/jb.92.3.597-600.1966.
5
A new gene cluster in yeast: the fatty acid synthetase system.酵母中的一个新基因簇：脂肪酸合成酶系统。
Hoppe Seylers Z Physiol Chem. 1971 Mar;352(3):377-84. doi: 10.1515/bchm2.1971.352.1.377.
6
Genetic effects of N-methyl-N'-nitro-N-nitrosoguanidine in Neurospora crassa.N-甲基-N'-硝基-N-亚硝基胍对粗糙脉孢菌的遗传效应。
Mol Gen Genet. 1970;106(3):195-207. doi: 10.1007/BF00340379.
7
New markers and map sequences in Neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.粗糙脉孢菌中的新标记和图谱序列，以及通过重复覆盖进行图谱绘制和用于测试连锁的多个易位株系的描述。
Genetica. 1969;40(3):247-78. doi: 10.1007/BF01787357.
8
Saturated fatty acid mutants in yeast.酵母中的饱和脂肪酸突变体。
Mol Gen Genet. 1971;113(1):1-19. doi: 10.1007/BF00335003.
9
Fatty acid replacements in a fatty acid auxotroph of Escherichia coli.大肠杆菌脂肪酸营养缺陷型中的脂肪酸替代物。
J Bacteriol. 1968 May;95(5):1658-65. doi: 10.1128/jb.95.5.1658-1665.1968.
10
Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase.缺乏β-羟基癸酰硫酯脱水酶的大肠杆菌脂肪酸突变体。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579-86. doi: 10.1073/pnas.58.4.1579.

粗糙脉孢菌的不饱和脂肪酸突变体。

Unsaturated fatty acid mutants of Neurospora crassa.

作者信息

Scott W A

出版信息

J Bacteriol. 1977 Jun;130(3):1144-8. doi: 10.1128/jb.130.3.1144-1148.1977.

DOI:10.1128/jb.130.3.1144-1148.1977
PMID:140860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235338/
Abstract

Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group V. Unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond position as well as different steric configurations were tested for growth requirements.

摘要

用N-甲基-N'-硝基-N-亚硝基胍处理粗糙脉孢菌的分生孢子，然后在含有悬浮于聚山梨醇酯NP-40中的多不饱和脂肪酸的培养基上进行分离，从而获得了不饱和脂肪酸（ufa）营养缺陷型。从与野生型杂交中重新分离得到的24个突变体被分为两个互补类，ufa-1和ufa-2，它们位于连锁群V上。测试了具有不同程度不饱和度、链长、双键位置以及不同空间构型的不饱和脂肪酸的生长需求。