一种分析DNA酶I足迹数据的新方法及其在TFIIIA/5S DNA复合物中的应用。
A new approach to the analysis of DNase I footprinting data and its application to the TFIIIA/5S DNA complex.
作者信息
Fairall L, Rhodes D
机构信息
MRC Laboratory of Molecular Biology, Cambridge, UK.
出版信息
Nucleic Acids Res. 1992 Sep 25;20(18):4727-31. doi: 10.1093/nar/20.18.4727.
Abstract
We have re-examined DNase I footprinting data for the binding of transcription factor IIIA (TFIIIA) to the 5S RNA gene, taking into account the protein-DNA contacts observed in the crystal structure of the DNase I/DNA complex (1, 2). This structure was not available when many of the original footprinting experiments on the TFIIIA/DNA complex were performed. In this way the pattern of DNase I cleavage can be interpreted to map out with greater precision the regions on the 5S DNA occupied by TFIIIA. Then, assuming the binding site for a zinc-finger may be the same as that found in the structure of the zinc-finger protein Zif268/DNA complex (3), and taking into account footprinting data for truncated forms of TFIIIA, the TFIIIA zinc-fingers were fitted within the permitted regions. On the basis of this, an alignment of the zinc-fingers of TFIIIA with its DNA binding site is proposed, which combines features of earlier models (4).
摘要
我们重新审视了转录因子IIIA（TFIIIA）与5S RNA基因结合的DNA酶I足迹数据，同时考虑了在DNA酶I/DNA复合物晶体结构中观察到的蛋白质-DNA接触情况（1, 2）。在对TFIIIA/DNA复合物进行许多最初的足迹实验时，这种结构尚未获得。通过这种方式，DNA酶I切割模式可以被解释，从而更精确地绘制出TFIIIA在5S DNA上占据的区域。然后，假设锌指的结合位点可能与锌指蛋白Zif268/DNA复合物结构中发现的位点相同（3），并考虑TFIIIA截短形式的足迹数据，将TFIIIA锌指拟合到允许的区域内。基于此，提出了TFIIIA锌指与其DNA结合位点的比对，该比对结合了早期模型的特征（4）。
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