Schettini G, Ventra C, Florio T, Grimaldi M, Meucci O, Scorziello A, Postiglione A, Marino A
Department of Human Communicative Science, II School of Medicine, Naples, Italy.
Pharmacol Biochem Behav. 1992 Sep;43(1):139-51. doi: 10.1016/0091-3057(92)90650-5.
The behavioral effects of the acute and subchronic administration of L-alpha-glycerylphosphorylcholine (alpha-GPC) on passive and active avoidance behavioral tasks were investigated. When administered IP after training together with scopolamine 2 h before retest, alpha-GPC reverses the scopolamine-induced amnesia in the passive avoidance conditioning in young and old rats. Furthermore, the subchronic treatment with alpha-GPC positively and significantly influences the performance of both young and old animals in the active avoidance test. Moreover, in in vitro/ex vivo experiments alpha-GPC potentiates receptor-stimulated phosphatidylinositol hydrolysis in cortical synaptoneurosomes derived from young and old animals. In young but not old animals, alpha-GPC significantly potentiates potassium (40 mM)-stimulated intrasynaptosomal calcium oscillations in purified synaptosomes derived from the hippocampus. These results show that alpha-GPC improves the performance of animals in both active and passive conditioning tasks. Furthermore, subchronic treatment with the compound enhances in young and restores in aged animals the transduction of the signal, namely, the receptor-mediated production of inositol phosphate and the potassium-induced calcium mobilization. These modifications may represent at least part of the molecular mechanism of action of the compound.
研究了急性和亚慢性给予L-α-甘油磷酸胆碱（α-GPC）对被动和主动回避行为任务的行为学影响。在训练后腹腔注射给药，并在重新测试前2小时皮下注射东莨菪碱，α-GPC可逆转东莨菪碱诱导的年轻和老年大鼠被动回避条件反射中的失忆。此外，α-GPC的亚慢性治疗对年轻和老年动物在主动回避测试中的表现有积极且显著的影响。此外，在体外/离体实验中，α-GPC可增强来自年轻和老年动物的皮质突触神经小体中受体刺激的磷脂酰肌醇水解。在年轻而非老年动物中，α-GPC可显著增强来自海马体的纯化突触体中钾（40 mM）刺激的突触体内钙振荡。这些结果表明，α-GPC可改善动物在主动和被动条件反射任务中的表现。此外，该化合物的亚慢性治疗可增强年轻动物并恢复老年动物的信号转导，即受体介导的肌醇磷酸生成和钾诱导的钙动员。这些改变可能至少代表了该化合物作用的部分分子机制。
