维托平Ti质粒pTiS4的物理图谱。
Physical map of the vitopine Ti plasmid pTiS4.
作者信息
Gérard J C, Canaday J, Szegedi E, de la Salle H, Otten L
机构信息
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
出版信息
Plasmid. 1992 Sep;28(2):146-56. doi: 10.1016/0147-619x(92)90045-c.
Within the Agrobacterium vitis group the vitopine strains represent a special subclass. Vitopine bacteria carry Ti plasmids with little or no homology with the well-characterized T-DNAs of Agrobacterium tumefaciens or Agrobacterium rhizogenes. The 262-kb Ti plasmid of the vitopine strain S4 was cloned and mapped. Homology studies with the octopine Ti plasmid pTiAch5, the nopaline Ti plasmid pTiC58, and the agropine/mannopine Ri plasmid pRiHRI identified several regions of homology. The origin of replication was localized to within 2.5 kb.
在葡萄土壤杆菌菌群中，维托品菌株代表一个特殊的亚类。维托品细菌携带的Ti质粒与根癌土壤杆菌或发根土壤杆菌中已充分表征的T-DNA几乎没有同源性。维托品菌株S4的262 kb Ti质粒被克隆并绘制图谱。与章鱼碱Ti质粒pTiAch5、胭脂碱Ti质粒pTiC58以及农杆碱/甘露碱Ri质粒pRiHRI的同源性研究确定了几个同源区域。复制起点定位于2.5 kb范围内。
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