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一种章鱼碱型Ri质粒的限制酶切图谱及其与Ti质粒的同源性

Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids.

作者信息

Jouanin L

出版信息

Plasmid. 1984 Sep;12(2):91-102. doi: 10.1016/0147-619x(84)90055-6.

DOI:10.1016/0147-619x(84)90055-6
PMID:6095353
Abstract

The Ri plasmid of an agropine-type Agrobacterium rhizogenes, strain HRI, was cloned in a cosmid and mapped with the restriction endonucleases BamHI, EcoRI, KpnI, SmaI, and XbaI. This plasmid is almost identical to pRi1855 and pRiA4b. A study by Southern hybridizations of the homologies with octopine pTiB6806 and nopaline pTiC58 makes it possible to propose the localization of certain functions on this plasmid, such as virulence, agropine catabolism, agropine synthesis, and the origin of replication.

摘要

发根农杆菌农杆碱型菌株HRI的Ri质粒被克隆到一个黏粒中,并用限制性内切酶BamHI、EcoRI、KpnI、SmaI和XbaI进行图谱分析。该质粒与pRi1855和pRiA4b几乎相同。通过与章鱼碱型pTiB6806和胭脂碱型pTiC58进行Southern杂交同源性研究,有可能确定该质粒上某些功能的定位,如毒性、农杆碱分解代谢、农杆碱合成和复制起点。

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