Yokoyama K, Itoman M, Takagishi K, Yamamoto M
Department of Orthopedic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
Plast Reconstr Surg. 1992 Nov;90(5):890-8. doi: 10.1097/00006534-199211000-00026.
We examined simple cold preservation of rat limbs in Euro-Collins' solution to elucidate the protective effect of coenzyme Q10 (CoQ10) on the ischemia-induced reperfusion injury in an ischemic extremity replant model. A total of 126 Lewis rat limb replants were performed. Limbs were amputated from donor rats and preserved at 4 degrees C in Euro-Collins' solution and were orthotopically grafted to isogeneic rats by microsurgical technique. In the experimental groups (n = 42), coenzyme Q10 (10 mg/kg) was injected intraperitoneally into the recipients about 1 hour before reperfusion. In the control groups (n = 84), the same dose of solvent was given by the same route. We evaluated vascular patency of anastomoses by direct observation or microangiogram and performed histologic examinations 7 days after replantation. In the control groups, the ischemic limit was 96 hours. Ischemic limbs treated with coenzyme Q10 showed a statistically significant (p < 0.05) improvement in vascular patency after 72 and 96 hours of ischemia. Histologically, bone viability with osteoblastic activity was maintained in coenzyme Q10-treated animals of the 72-hour ischemic group. We conclude that the protective effect of coenzyme Q10 on reperfusion injury is suggested in this replant model.
我们研究了在欧洲柯林斯溶液中对大鼠肢体进行简单冷藏，以阐明辅酶Q10（CoQ10）在缺血肢体再植模型中对缺血诱导的再灌注损伤的保护作用。总共进行了126例Lewis大鼠肢体再植手术。从供体大鼠身上截肢后，将肢体在4℃下保存在欧洲柯林斯溶液中，并通过显微外科技术原位移植到同基因大鼠身上。在实验组（n = 42）中，在再灌注前约1小时向受体腹腔注射辅酶Q10（10 mg/kg）。在对照组（n = 84）中，通过相同途径给予相同剂量的溶剂。我们通过直接观察或微血管造影评估吻合口的血管通畅情况，并在再植后7天进行组织学检查。在对照组中，缺血极限为96小时。用辅酶Q10处理的缺血肢体在缺血72小时和96小时后，血管通畅情况有统计学意义的改善（p < 0.05）。组织学上，在72小时缺血组接受辅酶Q10处理的动物中，具有成骨细胞活性的骨活力得以维持。我们得出结论，在这个再植模型中提示了辅酶Q10对再灌注损伤的保护作用。
