用于某些冷冻食品中肠球菌分离和计数的直接平板接种培养基的比较
COMPARISON OF DIRECT PLATING MEDIA FOR THE ISOLATION AND ENUMERATION OF ENTEROCOCCI IN CERTAIN FROZEN FOODS.
作者信息
BURKWALL M K, HARTMAN P A
出版信息
Appl Microbiol. 1964 Jan;12(1):18-23. doi: 10.1128/am.12.1.18-23.1964.
Abstract
A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate medium of Reinbold appeared to be the better of the low yield-high selectivity type of medium. Sodium carbonate (optimal concentration, 0.20%) was found to increase recovery substantially when added to certain media, especially in the presence of 0.05% Tween 80. When these two ingredients were incorporated into a medium modified after Slanetz and Bartley, the resultant medium was superior to other media for the isolation and enumeration of enterococci in certain frozen foods, such as peas and hamburger, by the direct plating method.
摘要
共对15种琼脂培养基进行了产量、选择性、可读性以及制备和使用的简易性方面的检测。巴恩斯铊培养基被选为高产-中等选择性类型培养基中的较好者，而莱因博尔德叠氮柠檬酸盐培养基似乎是低产-高选择性类型培养基中的较好者。发现碳酸钠（最佳浓度为0.20%）添加到某些培养基中时可显著提高回收率，尤其是在存在0.05%吐温80的情况下。当将这两种成分加入到经斯拉内茨和巴特利改良的培养基中时，所得培养基在通过直接平板接种法对某些冷冻食品（如豌豆和汉堡）中的肠球菌进行分离和计数方面优于其他培养基。
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