毛霉属、根霉属、根毛霉属、伞枝犁头霉和总状共头霉的特定菌株形成抗原性细胞外多糖。

Formation of antigenic extracellular polysaccharides by selected strains of Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum.

作者信息

De Ruiter G A, Van Bruggen-van der Lugt A W, Nout M J, Middelhoven W J, Soentoro P S, Notermans S H, Rombouts F M

机构信息

Wageningen Agricultural University, Department of Food Science, The Netherlands.

出版信息

Antonie Van Leeuwenhoek. 1992 Oct;62(3):189-99. doi: 10.1007/BF00582579.

DOI:10.1007/BF00582579

PMID:1416915

Abstract

In this study, polyclonal IgG antibodies raised against extracellular polysaccharides (EPS) of Mucor racemosus were characterised as almost specific for moulds belonging to the order of Mucorales. Cross-reactivity in the ELISA could be observed only towards the yeast Pichia membranaefaciens. EPS were isolated from various cultures of M. hiemalis growing on six different carbon sources and two nitrogen sources, with ratios varying from 0.13 to 0.44 relative to the amount of biomass. Other strains including Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum also excreted EPS, with ratios varying from 0.05 to 0.23. In all cases, the excreted EPS had similar antigenic properties as determined by ELISA. No enzymatic degradation of the antigenic parts of the polysaccharides could be observed upon prolonged incubation. Considering that all tested strains formed similar amounts of antigenic EPS there might be scope for the specific detection of biomass of Mucoralean moulds using ELISA techniques for example in food.

摘要

在本研究中，针对总状毛霉胞外多糖（EPS）产生的多克隆IgG抗体被鉴定为几乎对毛霉目霉菌具有特异性。在酶联免疫吸附测定（ELISA）中，仅观察到与膜醭毕赤酵母存在交叉反应。从在六种不同碳源和两种氮源上生长的冬生毛霉的各种培养物中分离出EPS，相对于生物量，其比例在0.13至0.44之间变化。包括毛霉属、根霉属、根毛霉属、伞枝犁头霉和总状共头霉在内的其他菌株也分泌EPS，比例在0.05至0.23之间变化。在所有情况下，通过ELISA测定，分泌的EPS具有相似的抗原特性。长时间孵育后未观察到多糖抗原部分的酶促降解。考虑到所有测试菌株产生的抗原性EPS量相似，例如在食品中，使用ELISA技术特异性检测毛霉目霉菌的生物量可能具有可行性。