通过酶联免疫吸附测定法检测曲霉属和青霉属细胞外多糖（EPS）：使用针对酸水解EPS产生的抗体。
Detection of Aspergillus and Penicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS.
作者信息
Kamphuis H J, De Ruiter G A, Veeneman G H, van Boom J H, Rombouts F M, Notermans S H
机构信息
Department of Food Science, Wageningen Agricultural University, The Netherlands.
出版信息
Antonie Van Leeuwenhoek. 1992 May;61(4):323-32. doi: 10.1007/BF00713940.
Species of the fungal genera Aspergillus and Penicillium produce immunologically active extracellular polysaccharides (EPS) in which galactofuranose residues are immunodominant. The antigenic determinant of the EPS of A. fumigatus, A. niger and P. digitatum could be removed by acid hydrolysis. Due to the hydrolysis of the EPS the immunological reaction between IgG anti-native EPS and hydrolysed EPS disappeared. Antibodies raised in rabbits against the acid hydrolysed EPS revealed new antigenic determinants that were exposed as a result of the acid hydrolysis. Immunological inhibitory experiments showed that the antibodies were no longer directed to galactofuranose residues. Enzyme Linked Immunosorbent Assay, carried out with antibodies raised against the acid hydrolysed EPS showed that the antibodies against the acid hydrolysed EPS were more species specific in comparison with the antibodies against the native EPS.
曲霉属和青霉属真菌能产生具有免疫活性的细胞外多糖（EPS），其中半乳呋喃糖残基具有免疫主导性。烟曲霉、黑曲霉和指状青霉EPS的抗原决定簇可通过酸水解去除。由于EPS发生水解，抗天然EPS的IgG与水解后的EPS之间的免疫反应消失。用酸水解后的EPS免疫家兔产生的抗体显示出因酸水解而暴露的新抗原决定簇。免疫抑制实验表明，这些抗体不再针对半乳呋喃糖残基。用酸水解后的EPS免疫产生的抗体进行酶联免疫吸附测定，结果显示，与抗天然EPS的抗体相比，抗酸水解EPS的抗体具有更强的种属特异性。
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